The importance of vitellogenin receptors in the oviposition of the pond wolf spider, Pardosa pseudoannulata  

作　　者：Zhi-Ming Yang Tian-Yu Lu Yong Wu Na Yu Guang-Ming Xu Qian-Qian Han Ze-Wen Liu 

机构地区：[1]Key Laboratory of Integrated Management of Crop Diseases and Pests(Ministry of Education),College of Plant Protection,Nanjing Agricultural University,Nanjing,210095 China

出　　处：《Insect Science》2022年第2期443-452,共10页昆虫科学（英文版）

基　　金：supported by the Fundamental Research Funds for the Central Universities(grant number:KJQN201809);the National Natural Science Foundation(grant number:31772185,31972296).

摘　　要：Vitellogenin receptor(VgR)is crucial for vitellogenin(Vg)uptake by oocytes.VgR is less known in Arachnida,especially in spiders.Different from only one VgR in an arthropod species,two VgRs,VgR-1 and VgR-2,were found in the pond wolf spider,Pardosa pseudoannulata.Both VgRs had the typical domains of the low-density lipoprotein receptor family except for the absence of the ligand-binding domain 1 in VgR-2.Spatiotemporal expression profiles showed that two VgR genes were consistently highly expressed in females and their ovaries,but VgR-1 was 48-fold that of VgR-2 in ovaries.The transcriptional level of VgR-1 was significantly downregulated by RNAi,but it did not work for VgR-2 although several trials were performed.Vg-1 and Vg-2 might be the ligands of VgR-1 because their expressions were also decreased in the dsVgR-1-treated females.Silencing VgR-1 prolonged the pre-oviposition period by 56 h.The expression of VgRs and Vgs were upregulated by juvenile hormones(JHs),which suggested that JHs were the essential factors to vitellogenesis in the spider.The present study revealed the importance of VgR-1 in the spider oviposition,which will improve the understanding on VgR physiological functions in spiders.

关 键 词：juvenile hormone Pardosa pseudoannulata REPRODUCTION vitellogenin receptor 

分 类 号：Q958[生物学—动物学]