Involvement of CYP347W1 in neurotoxin 3-nitropropionic acid-based chemical defense in mustard leaf beetle Phaedon cochleariae  

作　　者：Nanxia Fu Tobias Becker Wolfgang Brandt Maritta Kunert Antje Burse Wilhelm Boland 

机构地区：[1]Department of Bioorganic Chemistry,Max Planck Institute for Chemical Ecology,Jena,Germany [2]Key Laboratory of Tea Biology and Resource Utilization,Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou,China [3]Department of Bioorganic Chemistry,Leibniz Institute of Plant Biochemistry,Halle(Saale),Germany [4]Department of Natural Product Biosynthesis,Max Planck Institute for Chemical Ecology,Jena,Germany [5]Department of Medical Technology and Biotechnology,Ernst Abbe Hochschule Jena,Jena,Germany

出　　处：《Insect Science》2022年第2期453-466,共14页昆虫科学（英文版）

基　　金：supported by the Max Planck Society and the China Scholarship Council(grant number 201406300098).

摘　　要：Chrysomelina beetlesstore 3-nitropropionic acid in form of a pretoxin,isoxazolin-5-one glucoside-conjugated ester,to protect themselves against predators.Here we identified a cytochrome P450 monooxygenase,CYP347W1,to be involved in the production of the 3-nitropropionic acid moiety of the isoxazolin-5-one glucoside ester.Knocking down CYP347W1 led to a significant depletion in the concentration of the isoxazolin-5-one glucoside ester and an increase in the concentration of the isoxazolin-5-one glucoside in the larval hemolymph.Enzyme assays with the heterologously expressed CYP347W1 showed freeβ-alanine was not the direct substrate.Homology modeling indicated thatβ-alanine-CoA ester can fit into CYP347W1’s active site.Furthermore,we proved that Phaedon cochleariae eggs are not able to de novo synthesize 3-NPA,although both isoxazolin-5-one glucoside and its 3-NPA-conjugated ester are present in the eggs.These results provide direct evidence for the involvement of CYP347W1 in the biosynthesis of a P.cochleariae chemical defense compound.

关 键 词：chemical defense isoxazolin-5-one glucoside isoxazolin-5-one glucoside ester leaf beetles P450 3-nitropropionic acid(3-NPA) 

分 类 号：Q96[生物学—昆虫学]