Programming cell entry of molecules via reversible synthetic DNA circuits on cell membrane  

作　　者：Jiangbo Liu Yirong Chen Fan Li Xiuhai Mao Min Li Xiaolei Zuo Chunhai Fan 

机构地区：[1]Institute of Molecular Medicine,Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127,China [2]School of Chemistry and Chemical Engineering,Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine,Shanghai Jiao Tong University,Shanghai 200240,China

出　　处：《Fundamental Research》2021年第6期747-751,共5页自然科学基础研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grants No.22025404,21904086).

摘　　要：Cellular uptake of biomolecules is crucial for regulating cell function.However,powerful and biocompatible tools for dynamically manipulating the cell entry of single-stranded DNAs(ssDNAs)remain elusive.Herein,we constructed synthetic DNA circuits on the cell membrane to program the cell entry of ssDNAs,using toehold-mediated DNA strand displacement reactions.We found that the dimerization and trimerization of cholesterol-ssDNAs enhanced membrane-anchoring and cellular uptake of ssDNAs.Moreover,we demonstrated that de-dimerization and de-trimerization of cholesterol-ssDNAs could be accomplished by inputting recovery ssDNAs into the synthetic DNA circuits,which could simultaneously decrease the cellular uptake of ssDNAs.We speculate that operating the synthetic DNA circuits on the cell membrane will be a powerful strategy for regulating the cellular uptake of exogenous materials,which has important implications for bioimaging,drug delivery,and gene therapy.

关 键 词：Reversible DNA circuits Cholesterol-ssDNAs Dimerization Trimerization Cellular uptake 

分 类 号：Q2-3[生物学—细胞生物学]