bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis  

作　　者：Juan Gao Mei-ing Wang Jing-Jing Wang Hai-Ping Lu Jian-Xiang Liu 

机构地区：[1]State Key Laboratory of Plant Physiology and Biochemistry,College of Life Sciences,Zhejiang University,Hangzhou 310027,China

出　　处：《aBIOTECH》2022年第1期1-11,共11页生物技术通报（英文版）

基　　金：grants from the National Natural Science Foundation of China(Grant Nos.31872653 and 31800210);the Zhejiang Provincial Talent Program(Grant No.2019R52005);the Fundamental Research Funds for the Zhejiang Provincial Universities(Grant No.2021 XZZX023).

摘　　要：High temperature elicits a well-conserved response called the unfol ded protein response(UPR)to bring protein homeostasis in the endoplasmic reticulum(ER).Two key UPR regulators bZIP28 and bZIP60 have been shown to be essential for maintaining fertility under heat stress conditions in Arabidopsis,however,the function of transcriptional activator bZIP17,a paralog of bZIP28,in heat stress response at reproductive stage is not reported.Here we found that bzip17 mutant plants were sensitive to heat stress in terms of silique length and fertility comparing to that of wildtype(WT)Arabidopsis plants,and transcri ptomic analysis showed that 1380 genes were specifically up-regulated and 493 genes were specifically down-regulated by heat stress in the flowers of WT plants comparing to that in bzip17 mutant plants.These bZIP17-dependent up-regulated genes were enriched in responses to a biotic stresses such as water deprivation and salt stress.Further chromatin immuno-preci pitation coupled with high-throughput sequencing(ChIP-Seq)uncovered 1645 genes that were direct targets of bZIP17 in MYC-bZIP17 expressing seedlings subjected to heat stress.Among these 1645 genes,ERSE-II cis-element was enriched in the binding peaks of their promoters,and the up-regulation of 113 genes by heat stress in flowers was dependent on bZIP17.Our results revealed direct targets of bZIP17 in flowers during heat stress responses and demonstrated the important role of bZIP17 in maintai ning fertility upon heat stress in plants.

关 键 词：ARABIDOPSIS bZIP17 FLOWER FERTILITY Heat Stress UPR 

分 类 号：Q94[生物学—植物学]