玉米组蛋白编码基因鉴定与表达分析  

Identification and expression analysis of maize histone-coding genes

作　　者：马宇馨 李永国 张玮煜 朱倩洁 周帆[1] 邢继红[1] 张康 董金皋[1] MA Yuxin;LI Yongguo;ZHANG Weiyu;ZHU Qianjie;ZHOU Fan;XING Jihong;ZHANG Kang;DONG Jingao(State Key Laboratory of North China Crop Improvement and Regulation/Hebei Key Laboratory of PlantPhysiology and Molecular Pathology,Hebei Agricultural University,Baoding 071000,China;Experimental Farmof Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]华北作物改良与调控国家重点实验室/河北省植物生理与分子病理学重点实验室,河北农业大学,河北保定071000 [2]河北农业大学教学实验农场,河北保定071000

出　　处：《河北农业大学学报》2022年第2期51-58,共8页Journal of Hebei Agricultural University

基　　金：国家自然科学基金(3190186);河北省自然科学基金(C2019204141);国家玉米产业技术体系(CARS-02);省属高校基本科研业务费研究项目(KY2021043,KY2021044).

摘　　要：为了鉴定玉米基因组中组蛋白编码基因并分析其在不同条件下的表达规律,本研究利用生物信息学方法对玉米基因组中组蛋白编码基因进行了系统性鉴定和分析,利用同源性分析鉴定玉米组蛋白编码基因,利用保守结构域分析对组蛋白进行结构域鉴定,利用转录组测序数据对组蛋白编码基因进行表达规律分析。结果表明,玉米基因组中存在16个H2A,13个H2B,12个H3,9个H4和4个H1;对蛋白结构分析时发现玉米组蛋白保守结构域的数量和序列在同一亚族中高度保守,并且在不同物种中也非常保守;对玉米组蛋白基因的组织表达特异进行分析,发现玉米组蛋白编码基因在不同组织中表达水平有明显差异;对生物胁迫和非生物胁迫数据分析,发现组蛋白编码基因在不同胁迫下表达水平发生明显变化。本研究全基因组层面对玉米组蛋白编码基因进行了系统性鉴定和分析,明确了玉米中组蛋白的编码基因,揭示了组蛋白编码基因在玉米不同组织及生物和非生物胁迫中的表达规律,为进一步阐明其功能奠定了重要的理论基础。This study systematically identified histone-coding genes in the maize genome whose conservative domain were analyzed and expression patterns were drawn according to transcriptome data.The results showed that there were 16,13,12,9 and 4 genes encodeing H2A,H2B,H3,H4 and H1respectively in the maize genome.Protein structure analysis identified highly conserved domains in the same histone subfamily.The expression levels of maize histone genes in different tissues were significantly different,especially in germinated seeds where the expression level was highest.It was found that the expression of histone genes changed significantly under different stresses.Different expression in different physiological states were also observed.The identification and expression of maize histone genes in different tissues and biotic and abiotic stresses laid an important theoretical foundation for further elucidating their function.

关 键 词：玉米 组蛋白 结构域 生物信息学 基因表达 

分 类 号：S432.1[农业科学—植物病理学]