髓过氧化物酶-抗中性粒细胞胞质抗体相关性血管炎患者血清IgG4及Th1/Th2细胞因子检测分析  

Detection and analysis of serum IgG4 and Th1/Th2 cytokines in patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis

作　　者：秦陈浩[1] 顾爱萍[1] 展富琴[1] 冯萍[1] 顾昊宇 杜鸿[1] QIN Chen-hao;GU Ai-ping;ZHAN Fu-qin;FENG Ping;GU Hao-yu;DU Hong(Department of Clinical Laboratory,The Second Affiliated Hospital of Soochow University,Suzhou 215004,Jiangsu,China)

机构地区：[1]苏州大学附属第二医院检验科,苏州215004

出　　处：《海军军医大学学报》2022年第3期341-345,共5页Academic Journal of Naval Medical University

摘　　要：目的了解髓过氧化物酶(MPO)-抗中性粒细胞胞质抗体相关性血管炎(AAV)患者血清中IgG4及辅助性T细胞(Th)1/Th2细胞因子的表达情况。方法收集苏州大学附属第二医院50例AAV患者及40例同期健康体检者的血清样本,采用免疫比浊法测定IgG水平,采用ELISA法测定IgG4水平,采用流式细胞术微球阵列(CBA)技术检测血清中Th1/Th2细胞因子的水平,比较两组样本上述指标的差异,分析不同指标之间的相关性。结果共收集到50例AAV患者血清样本,其中MPO-AAV患者40例(80.0%)。MPO-AAV组血清IgG4水平为(1.13±0.33)g/L,IgG4/IgG比值为0.094±0.032,IL-4水平为(2.54±0.68)pg/mL,IL-6水平为15.22(8.83,59.52)pg/mL,均高于健康体检者[IgG4水平为(0.80±0.27)g/L,IgG4/IgG比值为0.070±0.028,IL-4水平为(1.96±0.60)pg/mL,IL-6水平为4.95(2.82,13.25)pg/mL],差异均有统计学意义(P均<0.01),且MPOAAV患者血清IgG4水平与IL-6水平呈正相关(r_(s)=0.508,P<0.05)。结论我院AAV患者中MPO-AAV比例较高。MPO-AAV患者血清中IgG4水平及IL-4、IL-6水平均升高,且IgG4升高与IL-6升高呈正相关,提示IgG4的升高与MPO-AAV患者Th1/Th2细胞因子平衡失调有关。Objective To investigate the expression of immunoglobulin(Ig)G4 and helper T cell(Th)1/Th2 cytokines in the serum of patients with myeloperoxidase-anti-neutrophil cytoplasmic antibody-associated vasculitis(MPOAAV).Methods Serum samples of 50 AAV patients and 40 healthy controls who taking a physical examination in the same period were collected from The Second Affiliated Hospital of Soochow University.The levels of IgG and IgG4 were detected by immunoturbidimetry and enzyme-linked immunosorbent assay,respectively.The expression of Th1/Th2 cytokines in serum was detected by flow cytometry microsphere array(CBA).The differences of the above indexes between the 2 groups were observed,and the correlations between different indexes were analyzed.Results Serum samples were collected from all the 50 AAV patients,including 40 of MPO-AAV patients(80.0%).In the MPO-AAV group,the serum IgG4 level was(1.13±0.33)g/L,IgG4/IgG ratio was 0.094±0.032,IL-4 level was(2.54±0.68)pg/mL,and IL-6 level was 15.22(8.83,59.52)pg/mL,which were significantly higher than those in the control group(IgG4:[0.80±0.27]g/L,IgG4/IgG ratio:0.070±0.028,IL-4:[1.96±0.60]pg/mL and IL-6:4.95[2.82,13.25]pg/mL;all P<0.01),and there was a positive correlation between the serum IgG4 and IL-6 levels in the MPO-AAV patients(r_(s)=0.508,P<0.05).Conclusion The proportion of MPO-AAV patients is high in AAV patients in our hospital.The serum IgG4,IL-4 and IL-6 levels in MPO-AAV patients are significantly increased,and the level of IgG4 is positively correlated with the level of IL-6,suggesting that the increase of IgG4 is related to the imbalance of Th1/Th2 cytokines in MPO-AAV patients.

关 键 词：免疫球蛋白G4 免疫球蛋白G TH1/TH2细胞因子 抗中性粒细胞胞质抗体相关性血管炎 髓过氧化物酶 

分 类 号：R593.2[医药卫生—内科学]