基于红外纳米谱学技术的人类头发黑素体研究  

Apply infrared nano-spectroscopy to the study of human hair melanosomes

作　　者：鲁瑶 彭蔚蔚[1,2] 吉特 朱化春[1,2] 王杰 赵红卫[1,2] 廖泽坤 薛艳玲 陈敏[1,2] LU Yao;PENG Weiwei;JI Te;ZHU Huachun;WANG Jie;ZHAO Hongwei;LIAO Zekun;XUE Yanling;CHEN Min(Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China;Shanghai Synchrotron Radiation Facility,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201204,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海应用物理研究所,上海201800 [2]中国科学院上海高等研究院上海光源,上海201204 [3]中国科学院大学,北京100049

出　　处：《核技术》2022年第4期1-8,共8页Nuclear Techniques

基　　金：国家重点研发项目(No.2017YFA0402902);国家自然科学基金联合重点项目(No.U2032206)资助。

摘　　要：人类头发中的黑素体产生的黑色素赋予了头发颜色,同时具有许多复杂的生物学功能,研究黑素体在头发中的分布及化学性质有着重要的意义。利用光热诱导共振技术(Photothermal Induced Resonance,PTIR,也称Atomic Force Microscopy Infrared,AFM-IR)研究了头发切片中的黑素体,对黑素体在皮质中的分布进行了原位的表征,测量了黑素体的红外特征光谱,并在纳米尺度的空间分辨率下进行了红外化学成像。实验结果表明:黑素体存在于黑头发的皮质层中,观测到的黑素体为椭球形,长轴与短轴平均尺寸为423 nm和337 nm,长短轴比平均为1.26,黑素体在皮质层中的分布由中心到外层逐渐增多。探索黑素体在头发中的形貌分布和化学信息,可为更好地理解黑素体在头发颜色和生物功能中的参与提供了实验基础。[Background] As one of the most visible organs, human hair has received intense attention in both basic and applied research. Melanin, produced by the melanosomes in human hair, imparts hair color while serving many complex biological functions. Due to the small size of human hair melanosomes, it is difficult to observe and study the chemical properties of melanosomes. In previous study on melanosome, chemical treatment methods were often used to extract melanosomes, which would affect their chemical properties and structures. [Purpose] This study aims to apply infrared nano-spectroscopy to exploring the structure, distribution and chemical properties of melanosomes in hair. [Methods] Photothermal induced resonance(PTIR, also known as AFM-IR), a popular technique for infrared imaging and infrared spectroscopy measurements unrestricted by diffraction limit, was used to study melanosomes in hair slices. Synchrotron radiation infrared spectroscopy, together with AFM-IR were employed to characterize the distribution of melanosomes in the cortex in situ, and to measure the infrared characteristics and infrared chemical imaging of melanosomes spectroscopy at nanoscale spatial resolution. [Results] The experimental results show that there are melanosomes in black hair but not found in white hair. Melanosomes are ellipsoidal, and the average lengths of long axis and short axis are 423 nm and 337 nm, respectively. The average length-to-short axis ratio is 1.26. The distribution of melanosomes in black hair gradually increases from the center to the outer layer. [Conclusions]Exploring the distribution and chemical information of melanosomes in hair carried out in this study provides an experimental basis for a better understanding of the involvement of melanosomes in hair color and biological function.

关 键 词：红外纳米光谱 化学成像 黑素体 头发 

分 类 号：TL99[核科学技术—核技术及应用] O433.1[机械工程—光学工程]