应用同步辐射X射线多模态成像研究死亡延迟时间对脑组织染色的影响  

Effects of death delay time on brain tissue staining based on synchronous X-ray multimodal imaging

作　　者：燕鑫 蔡小青[1,3,4] 汤乔伟 周峰 诸颖 王丽华[1,3,4] 胡钧 YAN Xin;CAI Xiaoqing;TANG Qiaowei;ZHOU Feng;ZHU Ying;WANG Lihua;HU Jun(Division of Physical Biology,CAS Key Laboratory of Interfacial Physics and Technology,Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China;ShanghaiTech University,Shanghai 201210,China;The Interdisciplinary Research Center,Shanghai Synchrotron Radiation Facility,Zhangjiang Laboratory,Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院上海应用物理研究所中国科学院微观界面物理与探测重点实验室,上海201800 [2]上海科技大学,上海201210 [3]中国科学院上海高等研究院基础交叉研究中心张江实验室,上海201210 [4]中国科学院大学,北京100049

出　　处：《核技术》2022年第4期17-23,共7页Nuclear Techniques

基　　金：国家自然科学基金原创探索计划项目(No.82050005);国家自然科学基金优秀青年科学基金项目(No.22022410);上海市科委仪器项目(No.19142202400)资助。

摘　　要：用于科学研究的人脑组织通常由遗体捐赠来获取,然而死亡延迟时间(Post-mortem Delay,PMD)是影响人脑组织染色的重要因素之一,目前对于PMD如何影响人脑组织的染色尚缺乏研究。本工作利用取脑后放置不同时间的小鼠脑组织来模拟人脑PMD的状态,采用高尔基染色、尼氏染色对其大脑皮层区域染色并进行光学显微镜成像,重点运用同步辐射X射线微米CT成像技术研究PMD对小鼠脑组织高尔基染色的影响。结果表明:随着PMD的增长,神经元的完整性和复杂性呈现明显的降低趋势,神经元的树突棘的数目明显减少,同步辐射X射线微米CT成像的结果也证实了锥体神经元纤维结构随放置时间的延长逐渐变短直至消失。研究结果将为人脑组织高尔基染色方法的建立和同步辐射X射线成像奠定基础。[Background] Human brain tissue is usually obtained by donating, and post-mortem delay(PMD) is one of the important factors affecting Golgi staining in human brain. However, currently there are relatively few studies on how PMD affects Golgi staining in human brain tissue. [Purpose] This study aims to explore the changes in the morphology and structure of pyramidal neurons in the cerebral cortex of mice with PMD. [Method] The changes of human brain PMD were simulated by using mouse brain tissues placed in vitro for different times, and light microscope imaging data were obtained by Golgi staining and Nissl staining. At the same time, the synchrotron X-ray micro-CT imaging technology was used to realize neurons three-dimensional imaging visualization. The Sholl analysis was employed to study the Golgi-stained pyramidal neurons in the cerebral cortex. [Results] Analysis results show that the completeness and complexity emerge a significant decreasing trend with the increase of PMD, and the statistical results of dendritic spines also show the same decrease trend. The three-dimensional neuron visualization results reconstructed by synchrotron radiation X-ray micro-CT technology confirm the above analysis results.[Conclusions] This study provides a research foundation for the establishment of Golgi staining method of human brain tissue and synchrotron radiation X-ray imaging.

关 键 词：皮层锥体神经元 神经元结构 高尔基染色 同步辐射X射线微米CT技术 

分 类 号：TL99[核科学技术—核技术及应用]