原发性EB病毒感染患儿AIM2炎症小体通路的表达及与肝损伤的相关性  

Expression of AIM2 inflammasome signaling pathway in primary EBV infection in children and its correlation with liver injury

作　　者：金龙腾[1] 孙妍 黄基红[1] 温正旺[1] 石海矾[1] 陈益平[1] JIN Longteng;SUN Yan;HUANG Jihong;WEN Zhengwang;SHI Haifan;CHEN Yiping(Department of Childhood Infectious Diseases,the Second Affiliated Hospital&Yuying Children’s Hospital of Wenzhou Medical University,Wenzhou 325000,China)

机构地区：[1]温州医科大学附属第二医院育英儿童医院儿童感染科,浙江温州325000

出　　处：《温州医科大学学报》2022年第3期205-209,共5页Journal of Wenzhou Medical University

基　　金：温州市基础性科研项目(Y20190003,Y20210272)。

摘　　要：目的:探讨原发性EB病毒感染患儿黑素瘤缺乏因子2(AIM2)炎症小体通路的表达及与肝损伤的相关性。方法:纳入2020年1月至2021年6月82例原发性EB病毒感染住院患儿(入院时EB-VCA-Ig M和EB-DNA检测均阳性),按有无肝损伤分为肝功能异常组(52例)与肝功能正常组(30例);同期选取门诊体检EBVIgG阳性(EB-VCA-IgG、EB-NA-IgG)而EB-VCA-IgM阴性的50例健康儿童为既往EBV感染组;门诊体检EBV-IgG和EBV-VCA-Ig M均阴性的50例健康儿童为对照组。实时荧光定量PCR技术检测外周血单个核细胞AIM2炎症小体(AIM2、ASC和caspase-1)m RNA表达,酶联免疫吸附试验检测血清白细胞介素(IL)-1β、IL-18水平。结果:原发性EBV感染患儿外周血AIM2、ASC和caspase-1 mRNA的表达量以及IL-1β和IL-18水平均高于既往EBV感染组、对照组(P<0.05),并且血清EBV-DNA载量与AIM2、ASC和caspase-1 mRNA表达及IL-1β和IL-18水平呈正相关(r=0.712、0.494、0.479、0.384、0.300,P<0.05)。原发性EBV感染组中,肝功能异常组、肝功能正常组患儿AIM2、ASC、caspase-1 mRNA表达量以及IL-1β、IL-18水平均高于对照组,以肝功能异常组更高,差异均有统计学意义(P<0.05)。AMI2 mRNA与ASC mRNA、caspase-1 mRNA表达量以及IL-1β、IL-18水平均呈正相关(r=0.718、0.729、0.548、0.529,P<0.05)。结论:AIM2炎症小体通路可能参与了原发性EBV感染,并与EBV肝炎存在相关性。Objective: To explore the expression of absent in melanoma 2(AIM2) inflammatory signaling pathway in children with primary Epstein-Bark virus(EBV) infection and its correlation with liver injury.Methods: A total of 82 children with positive EB-VCA IgM and EBV-DNA from January 2020 to June 2021 were enrolled as primary EBV infection group, among whom 52 had liver injury and 30 had no liver injury;50 healthy children with positive EBV-IgG(EB-VCA-IgG, EB-NA-IgG) and negative EB-VCA-IgM as previous EBV infection group and 50 healthy children with negative EBV-IgG/EBV-VCA IgM as the healthy control group.Real-time PCR was used to measure the mRNA expression of AIM2 inflammasome(AIM2, ASC and caspase-1)in peripheral blood mononuclear cells. The serum interleuk in(IL)-1β, IL-18 level was detected by ELISA.Results: Compared with previous EBV infection group and the control group, primary EBV infection group had significantly higher relative mRNA expression level of AIM2, ASC, and caspase-1 in PBMCs and serum level of IL-1β and IL-18(P<0.05). The level of AIM2, ASC, caspase-1 mRNA in PBMCs and serum level of IL-1β, IL-18were positively correlated with the virus load in serum(r=0.712, 0.494, 0.479, 0.384, 0.300, P<0.05). Compared with the control group, both liver injury group and no liver injury group had significantly higher relative mRNA expression level of AIM2, ASC, and caspase-1 in PBMCs and serum level of IL-1β and IL-18(P<0.05). The liver injury group had significantly higher level of above-mentioned parameters than the no liver injury group(P<0.05).The relative expression level of AIM2 was positively correlated with the relative mRNA expression level of ASC and caspase-1 and the serum level of IL-1β and IL-18(r=0.718, 0.729, 0.548, 0.529, P<0.05). Conclusion:The AIM2 inflammasome signaling pathway might be involved in the pathogenesis of primary EBV infection in children and is associated with the EBV hepatitis.

关 键 词：EB病毒 AIM2炎症小体 肝损伤 

分 类 号：R72[医药卫生—儿科]