PRMT6 physically associates with nuclear factor Y to regulate photoperiodic flowering in Arabidopsis  

作　　者：Pingxian Zhang Xiulan Li Yifan Wang Weijun Guo Sadaruddin Chachar Adeel Riaz Yuke Geng Xiaofeng Gu Liwen Yang 

机构地区：[1]Biotechnology Research Institute,Chinese Academy of Agricultural Science,Beijing 100081,China [2]College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China [3]College of Life and Environmental Sciences,Minzu University of China,Beijing 100081,China

出　　处：《aBIOTECH》2021年第4期403-414,共12页生物技术通报（英文版）

基　　金：the Natural National Science Foundation of China(32101786);the National Transgenic Major Program(2019ZX08010-002);the Fu ndamental Research Funds for Central Non-profit Scientific Institution(1610392017001);the Baichuan Project at the College of Life Science and Technology,Huazhong Agricultural University.

摘　　要：The timing of floral transition is critical for reproductive success in flowering plants.In long-day(LD)plant Arabidopsis,the floral regulator gene FLOWERING LOCUS T(FT)is a major component of the mobile florigen.FT expression is rhythmically activated by CONSTANS(CO),and specifically accumu-lated at dusk of LDs.However;the underlying mechanism of adequate regulation of FT transcription in response to day-length cues to warrant flowering time still remains to be investigated.Here,we identify a homolog of human protein arginine methyltransferases 6(HsPRMT6)in Arabidopsis,and confirm AtPRMT6 physically interacts with three positive regulators of flowering Nuclear Factors YC3(NF-YC3),NF-YC9,and NF-YB3.Further investigations find that AtPRMT6 and its encoding protein accumulate at dusk of LDs.PRMT6-mediated H3 R2me2a modification enhances the promotion of NF-YCs on FT transcription in response to inductive LD signals.Moreover,AtPRMT6 and its homologues proteins AtPRMT4a and AtPRMT4b coordinately inhibit the expression of FLOWERING LOCUS C,a suppressor of FT.Taken together,our study reveals the role of arginine methylation in photoperiodic pathway and how the PRMT6-mediating H3R2me2a system interacts with NF-CO module to dynamically control FT expression and facilitate flowering time.

关 键 词：Arginine methylation Protein arginine methyltransferases 6 Nuclear factors Y Flowering time 

分 类 号：Q945[生物学—植物学]