浓缩生长因子干预大鼠骨髓间充质干细胞的增殖、成骨分化及迁移  

Effects of concentrated growth factors on the proliferation,osteogenic differentiation and migration of rat bone marrow mesenchymal stem cells

作　　者：汤凯 招文华 尚奇[1,2] 沈耿杨[2,3] 张志达[2,3] 何嘉辉 张鹏[1,2] 余富勇 陈桂锋 任辉[2,3] 江晓兵[2,3] 余翔[2,3] Tang Kai;Zhao Wenhua;Shang Qi;Shen Gengyang;Zhang Zhida;He Jiahui;Zhang Peng;Yu Fuyong;Chen Guifeng;Ren Hui;Jiang Xiaobing;Yu Xiang(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Lingnan Medical Research Center,Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China)

机构地区：[1]广州中医药大学,广东省广州市510405 [2]广州中医药大学岭南医学研究中心,广东省广州市510405 [3]广州中医药大学第一附属医院,广东省广州市510405

出　　处：《中国组织工程研究》2022年第31期4935-4939,共5页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金面上项目(81774338),项目负责人:江晓兵;广东省医学科研基金项目(A2021320),项目负责人:余翔;广州中医药大学青年培育项目(2019QNPY04),项目负责人:余翔。

摘　　要：背景:在研究骨髓间充质干细胞的增殖、成骨分化基础上,揭示其迁移机制是再生医学研究的热门课题之一。目的:探究浓缩生长因子对大鼠骨髓间充质干细胞增殖、成骨分化及迁移能力的影响。方法:大鼠心脏静脉取血,变速离心提取浓缩生长因子。体外培养大鼠骨髓间充质干细胞,分为实验组和对照组,实验组分别用含体积分数5%,10%,20%浓缩生长因子的α-MEM完全培养基进行培养,对照组用不含浓缩生长因子的α-MEM完全培养基进行培养。应用CCK-8法检测细胞增殖能力,划痕实验、Transwell迁移小室实验检测细胞迁移能力,茜素红染色检测细胞成骨分化能力。结果与结论:①不同体积分数的浓缩生长因子均能促进大鼠骨髓间充质干细胞增殖;②划痕实验显示实验组均能够促进大鼠骨髓间充质干细胞迁移,其中12 h时体积分数10%浓缩生长因子组迁移速度最快;Transwell迁移小室实验结果显示,实验组大鼠骨髓间充质干细胞迁移数目均高于对照组,且体积分数10%浓缩生长因子组的细胞迁移数量更多;③茜素红染色显示,实验组在7 d时钙结节数量均多于对照组;④结果表明,不同体积分数的浓缩生长因子均能够促进大鼠骨髓间充质干细胞的体外增殖、分化及迁移,其中体积分数10%浓缩生长因子具有较快的促进细胞迁移能力。BACKGROUND:On the basis of studying the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells,it is one of the hot topics in regenerative medicine to reveal their migration mechanism.OBJECTIVE:To investigate the effects of concentrated growth factor on proliferation,osteogenic differentiation,and migration of rat bone marrow mesenchymal stem cells.METHODS:Concentrated growth factor was extracted from rat heart vein by variable speed centrifugation.Rat bone marrow mesenchymal stem cells were cultured in vitro and divided into experimental and control groups.Cells in the experimental groups were cultured inα-MEM complete medium supplemented with 5%,10%and 20%concentrated growth factors by volume fraction,separately.Cells in the control group were cultured inα-MEM complete medium without concentrated growth factor.Cell proliferation was detected by CCK-8 assay.Cell migration was detected by scratch test and Transwell migration chamber assay.Osteogenic differentiation was detected by alizarin red staining.RESULTS AND CONCLUSION:(1)Concentrated growth factor with different volume fractions could promote the proliferation of rat bone marrow mesenchymal stem cells with different volume fractions.(2)The scratch test showed that the migration of rat bone marrow mesenchymal stem cells could be promoted in the experimental groups,and the migration rate of 10%concentrated growth factor group was fastest at 12 hours.Transwell migration chamber assay showed that the number of migrated rat bone marrow mesenchymal stem cells in the experimental groups was higher than that in the control group.The number of migrated rat bone marrow mesenchymal stem cells was highest in the 10%concentrated growth factor group.(3)Alizarin red staining showed that the total number of calcium nodules in the experimental groups was higher than that in the control group on day 7.(4)The results showed that all concentrated growth factors with different volume fractions could promote the proliferation,differentiation,and mi

关 键 词：浓缩生长因子 骨髓间充质干细胞 细胞增殖 成骨分化 细胞迁移 

分 类 号：R459.9[医药卫生—治疗学] R34[医药卫生—临床医学]