不同温度和饥饿胁迫下琥珀蚕AaHsp90基因的表达特征  

Expression Characteristics of AaHsp90 in Antheraea assama under Different Temperature and Starvation Stress

作　　者：杨伟克[1] 刘增虎[1] 唐芬芬 胡昌雄 董占鹏[1] YANG Weike;LIU Zenghu;TANG Fenfen;HU Changxiong;DONG Zhanpeng(Sericulture and Apiculture Research Institute,Yunnan Academy of Agricultural Sciences,Mengzi 661101,China;College of Biological and Agricultural Sciences,Honghe University,Mengzi 661101,China)

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 [2]红河学院生物科学与农学学院,云南蒙自661101

出　　处：《河南农业科学》2022年第2期133-138,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31360586);云南省基础研究计划面上项目(2022FB226);云南省现代农业蚕桑产业技术体系建设项目(2019KJTX006);红河学院博士专项(BS2003);云南省科技计划重点研发专项(202102AE090010)。

摘　　要：为了探究高温、低温和饥饿胁迫对琥珀蚕热激蛋白AaHsp90基因表达水平的影响,揭示琥珀蚕适应环境的分子机制,对5龄第3天琥珀蚕幼虫分别进行38℃高温、4℃低温和饥饿胁迫处理,以26℃正常饲养组为对照,采用实时荧光定量PCR检测分析中肠、脂肪体和血淋巴AaHsp90基因的相对表达量变化情况。结果表明,在测定的时间范围内,38℃高温处理后,中肠、脂肪体和血淋AaHsp90基因的表达量均呈现先明显增加,后随着处理时间的延长急剧减弱的趋势。4℃低温处理对琥珀蚕幼虫中肠、脂肪体和血淋巴Aahsp90基因的表达有一定的抑制作用。饥饿处理组中,琥珀蚕血淋巴、脂肪体和中肠AaHsp90基因在饥饿12 h和18 h呈现上调表达趋势,而在饥饿24 h时,AaHsp90基因的表达量急剧降低。综上可知,高温和饥饿胁迫能诱导琥珀蚕Aahsp90基因的表达,而低温胁迫主要抑制AaHsp90基因的表达,提示AaHsp90蛋白在琥珀蚕幼虫适应高温和饥饿胁迫的过程中发挥了重要作用。In order to investigate the influences of different temperature and starvation stress on the expression of AaHsp90 and reveal the molecular mechanism of adaptation to environment in Antheraea assama,with the normal feeding group at 26℃as the control,the expression change of AaHsp90 was detected by real-time PCR in midgut,fat body and hemlymph after high temperature stress at 38℃,low temperature stress at 4℃and starvation stress respectively for different time on the third day of the fifth larvae.Results showed that the expressions of AaHsp90 in midgut,fat body and hemlymph of Antheraea assama were increased obviously at first and then decreased sharply with the prolongation of treatment time at 38℃.There has a certain inhibitory effect on the expression of AaHsp90 in midgut,fat body and hemolymph after tretment with 4℃for different time.After treatment with starvation,the AaHsp90expressions were increased at 12 h and 18 h and decreased sharply at 24 h in midgut,fat body and hemolymph of Antheraea assama.Comprehensive analysis showed that high temperature and starvation stress could induce the expression of AaHsp90,while low temperature stress mainly suppressed its expression.The AaHsp90 protein in Antheraea assama may play an important role in the process of adaptation to high temperature and starvation stress.

关 键 词：琥珀蚕 热激蛋白90 温度胁迫 饥饿胁迫 表达特征 

分 类 号：S885.9[农业科学—特种经济动物饲养] Q966[农业科学—畜牧兽医]