隔药饼灸对动脉粥样硬化易损斑块兔RhoA/ROCK1信号通路的影响  

Effects of Medicinal Cake-separated Moxibustion on RhoA/ROCK1 Signaling Pathway in Rabbits with Atherosclerosis Vulnerable Plaques

作　　者：吴雪芬[1,2] 易丽贞 刘欣 宋家薇 饶泽华 廖意娟 马逸杰 岳增辉 WU Xuefen;YI Lizhen;LIU Xin;SONG Jiawei;RAO Zehua;LIAO Yijuan;MA Yijie;YUE Zenghui(College of Acupuncture&Moxibustion and Tui-na,Hunan University of Chinese Medicine,Changsha 410208,China;College of Acupuncture and Orthopedics,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208 [2]贵州中医药大学针灸骨伤学院,贵州贵阳550025

出　　处：《中国中医药信息杂志》2022年第4期80-86,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81874430)。

摘　　要：目的观察隔药饼灸对动脉粥样硬化(AS)易损斑块兔RhoA/ROCK1信号通路的影响,探讨其稳定AS易损斑块的作用机制。方法将雄性新西兰大耳白兔随机分为空白组和手术组,手术组采用高脂饲料喂养联合腹主动脉球囊损伤法建立AS易损斑块兔模型。将成模兔随机分为模型组、直接灸组、隔药饼灸组、西药组和抑制剂组,分别给予相应干预,连续8周。ELISA检测血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、载脂蛋白(APO)-A、APO-B含量,HE染色观察腹主动脉血管损伤程度,Western blot检测腹主动脉组织Ras同源物基因组成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶1(ROCK1)蛋白表达,RT-PCR检测腹主动脉组织RhoA和ROCK1 mRNA表达。结果与空白组比较,模型组兔血清TC、TG、LDL-C、APO-B含量显著增加,HDL-C、APO-A含量显著减少(P<0.01);腹主动脉内膜增厚,有斑块形成,斑块内可见多处出血,纤维帽薄,管腔狭窄,管腔内可见血栓;腹主动脉组织RhoA、ROCK1蛋白及mRNA表达显著升高(P<0.01)。与模型组比较,直接灸组、隔药饼灸组和西药组兔血清TC、TG、LDL-C、APO-B含量显著减少,HDL-C、APO-A含量显著增加(P<0.01,P<0.05);HE染色显示,直接灸组兔血管损伤程度未改善,隔药饼灸组和西药组兔血管中膜损伤程度稍减轻,3组均未见血栓形成,斑块稳定性较模型组增加;腹主动脉组织RhoA和ROCK1蛋白及mRNA表达显著降低(P<0.01,P<0.05)。结论隔药饼灸能稳定AS易损斑块,其机制可能与抑制RhoA/ROCK1信号通路有关。Objective To observe the effects of medicinal cake-separated moxibustion on the RhoA/ROCK1 signaling pathway of atherosclerosis vulnerable plaques rabbits;To explore the mechanism of stabilizing atherosclerosis vulnerable plaques.Methods Male New Zealand white rabbits were randomly divided into the blank group and operation group.A rabbit model of atherosclerosis vulnerable plaque was established by high-fat diet combined with abdominal aortic balloon injury in operation group.The model rabbits were randomly divided into model group,direct moxibustion group,medicinal cake-separated moxibustion group,Western medicine group and inhibitor group,and were given corresponding interventions for a total of 8 weeks of treatment.ELISA was used to detect the contents of TC,TG,LDL-C,HDL-C,APO-A and APO-B in serum.HE staining was used to observe the degree of abdominal aortic vascular damage.Western blot was used to detect the expressions of RhoA and ROCK1 protein in abdominal aortic tissue.RT-PCR was used to detect the expressions of RhoA and ROCK1 mRNA in abdominal aorta.Results Compared with the blank group,the contents of TG,TC,LDL-C and APO-B in serum of the rats in model group increased significantly,and the contents of HDL-C and APO-A decreased significantly(P<0.01).The abdominal aorta intima was thickened,the spots were formed,with multiple bleeding in the plaque,the fiber cap was thin,stenosis of the lumen,thrombus could be seen in the lumen.The expressions of RhoA and ROCK1 protein and mRNA in abdominal aorta tissue significantly increased(P<0.01).Compared with the model group,the contents of TC,TG,LDL-C and APO-B in serum of the rats in direct moxibustion group,medicinal cake-separated moxibustion group and Western medicine group were significantly reduced,the contents of HDL-C and APO-A significantly increased(P<0.01,P<0.05).HE staining showed that the degree of vascular injury in the direct moxibustion group did not improve,the degree of vascular media injury in the medicinal cake-separated moxibustion group and

关 键 词：隔药饼灸 动脉粥样硬化 易损斑块 RhoA/ROCK1信号通路 兔 

分 类 号：R245.8[医药卫生—针灸推拿学]