清肺利咽颗粒UPLC指纹图谱研究  

Study on UPLC fingerprint and simultaneous determination of twelve components in Qingfei Liyan granule

作　　者：江洁怡 黄华靖 杨敏娟 李素梅 李养学 毕晓黎 JIANG Jieyi;HUANG Huajing;YANG Minjuan;LI Sumei;LI Yangxue;BI Xiaoli(Guangdong Province Engineering and Technology Research Institute of Traditional Chinese Medicine,Guangzhou 510095,China;Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine,Guangzhou 510095,China;School of the Fifth Clinical Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广东省中医药工程技术研究院,广东广州510095 [2]广东省中医药研究开发重点实验室,广东广州510095 [3]广州中医药大学第五临床医学院,广东广州510405

出　　处：《广东药科大学学报》2022年第2期19-23,共5页Journal of Guangdong Pharmaceutical University

基　　金：广东省科技计划项目“创新中药研发平台建设”(2017A070701017)。

摘　　要：目的建立清肺利咽颗粒UPLC指纹图谱,为清肺利咽颗粒的质量控制提供方法和依据。方法采用超高效液相色谱法(UPLC),色谱柱Waters Cortecs T_(3)(2.1 mm×150 mm,1.6μm),以乙腈-0.1%磷酸为流动相,梯度洗脱,流速0.35 mL/min,柱温为25℃,检测波长为245 nm、280 nm。结果建立了清肺利咽颗粒UPLC指纹图谱,共标定了23个共有峰,并指认了其中12个共有峰,12批次清肺利咽颗粒的指纹图谱相似度均在0.9以上,聚类分析、主成分分析显示12批样品聚为2类。结论所建立的指纹图谱分析方法全面、稳定、可靠,可为清肺利咽颗粒的整体质量控制提供方法参考。Objective To establish a method of fingerprint for Qingfei Liyan granule by UPLC,which can provide a reference for the quality control of Qingfei Liyan granule.Methods UPLC method was adopted,which was performed on a Waters Cortecs T_(3)(2.1 mm×150 mm,1.6μm),with a mobile phase of acetonitrile-0.1%phosphoric acid by gradient elution at a flow rate of 0.35 mL/min,column temperature was 25℃,and detection wavelength was set at both 245 nm and 280 nm.Results The UPLC fingerprint of Qingfei Liyan granule was established,23 peaks were selected as the character fingerprint peaks,12 of which were identified.Good similarities were found in the established fingerprint through similarity analysis.Cluster analysis and principal component analysis showed that 12 batches of samples were clustered into 2 groups.Conclusion The established UPLC fingerprint of Qingfei Liyan granule is comprehensive,stable,and reliable,which provides a method reference for evaluating and controlling the overall quality of Qingfei Liyan granule.

关 键 词：清肺利咽颗粒 指纹图谱 质量评价 

分 类 号：R284.1[医药卫生—中药学]