接种木霉对本氏烟草生长及营养吸收的影响  

Effect of Inoculation with Trichoderma on the Growth and Nutrient Uptake of N.benthamiana

作　　者：李一晗 庄文化[1,2] 曾玥 谭霄 谭博 LI Yihan;ZHUANG Wenhua;ZENG Yue;TAN Xiao;TAN Bo(State Key Laboratory of Hydraulics and Mountain River Engineering,Chengdu 610065,China;College of Water Re-source and Hydropower,Sichuan University,Chengdu 610065,China)

机构地区：[1]水力学与山区河流开发保护国家重点实验室,四川成都610065 [2]四川大学水利水电学院,四川成都610065

出　　处：《安徽农学通报》2022年第7期36-39,共4页Anhui Agricultural Science Bulletin

基　　金：四川省科技厅重点研发项目(2019YFN0153);高校基本科研业务费(2021SCU12037)。

摘　　要：木霉对促进植物的生长发育具有重要作用,对现代农业生产至关重要,但植物的营养吸收和代谢过程对木霉的响应机制尚缺乏深入研究。为探究哈茨木霉对植物的促生作用及生理生化特性的影响,采用木霉孢子悬浮液灌根的方法进行本氏烟草盆栽试验,测算烟草株高、叶面积、叶片氮磷含量、叶绿素含量、抗氧化酶(POD和SOD)活性。结果表明,相比于对照组,木霉处理后的烟草株高、叶面积和地上干重分别增加47.06%、46.67%和26.23%;植物叶片氮、磷含量分别增加18.57%、34.94%;叶绿素a和叶绿素b含量分别增加19.86%、39.01%;过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性显著上升。因此,木霉通过促进土壤矿化,有利于植物营养吸收,显著增加植物的株高、叶面积,进而提高了光合速率和抗氧化酶的活性,提高了植物的抗逆性,最终提高了地上部分的干物质量积累。Trichoderma has played an important role in promoting plant growth and development,which is crucial for agricultural production,yet the mechanisms of plant nutrient uptake and metabolic processes in response to Tricho⁃derma are still lacking in-depth investigation.To explore the effect of Trichoderma harzianum on the plant′s growth promoting effect and physiological and biochemical traits,this study was conducted by using Trichoderma spore sus⁃pension for root irrigation in a potted experiment of N.benthamiana.Plant height,leaf area,leaf nitrogen and phos⁃phorus content,chlorophyll content,and antioxidant enzymes(POD and SOD)activities were measured.The results showed that the plant height,leaf area and above-ground dry weight of N.benthamiana with Trichoderma inoculation increased by 47.06%,46.67% and 26.23%,respectively;the nitrogen and phosphorus contents of leaves increased by 18.57% and 34.94%,respectively;the chlorophyll a and chlorophyll b contents increased by 19.86% and 39.01%,respectively;the activities of peroxidase(POD)and superoxide dismutase(SOD)were significantly in⁃creased.This study demonstrated that Trichoderma facilitated plant nutrient uptake and significantly increased plant height and leaf area by promoting soil mineralization.As a result,the photosynthetic rate and antioxidant enzyme ac⁃tivity increased,which enhanced plant stress tolerance and ultimately promoted the accumulation of dry matter in above-ground parts.

关 键 词：本氏烟草 哈茨木霉 灌根 促生效果 生理生化特性 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]