环境因素对淡水浮丝藻生长及产生嗅味的影响  

Effects of Environmental Factors on the Growth and 2-MIB Production of Planktothrix sp.

作　　者：成建国 马力通[1,2] 赵文渊 刘云颖 CHENG Jian-guo;MA Li-tong;ZHAO Wen-yuan;LIU Yun-ying(School of Chemistry and Chemical Engineering,Inner Mongolia University of Science and Technology,Baotou 014010,P.R.China;Inner Mongolia Engineering Research Center of Comprehensive Utilization of Bio-Coal Chemical Industry,Baotou 014010,P.R.China)

机构地区：[1]内蒙古科技大学化学与化工学院,内蒙古包头014010 [2]生物煤化工综合利用内蒙古自治区工程研究中心,内蒙古包头014010

出　　处：《水生态学杂志》2022年第2期70-76,共7页Journal of Hydroecology

基　　金：国家自然科学基金(21766025);内蒙古自治区科技计划(2020年度);中国科学院“西部之光”人才培养引进计划“西部青年学者”项目;内蒙古科技大学创新基金-优秀青年科学基金(2017YQL03);内蒙古科技大学创新基金(2019QDL-B20);内蒙古自治区教育厅,高等学校科学研究项目(NJZY21402)。

摘　　要：研究温度、营养盐浓度以及光照强度对淡水浮丝藻(Planktothrix sp.)及其代谢产物二-甲基异莰醇(2-MIB)的影响,为控制丝状蓝藻生长及预警水体嗅味物质污染提供实践依据。浮丝藻由中国科学院水生生物研究所淡水藻种库分离于安徽巢湖;设置的实验温度为15、20、25、30℃,BG-11营养盐溶液体积分数为20%、30%、50%、100%,光照为1000、2000、3000、4000 lx。结果表明,温度对浮丝藻生长密度、总2-MIB生成量及胞外2-MIB分泌量影响最显著,25℃是浮丝藻最适宜生长温度,最高细胞密度可达6.04×10^(5)个/mL,30℃是其最适宜的产2-MIB温度,总2-MIB生成量和胞外2-MIB分泌量均最高,分别为3.64×10^(3) ng/L和2.78×10^(3) ng/L;其次为营养盐浓度,增大BG-11营养盐浓度,浮丝藻生长密度和总2-MIB生成量会随之增加,低浓度营养盐时胞外2-MIB分泌量所占比例较高,表明低浓度营养盐更有利于浮丝藻进行胞外2-MIB分泌;光照对浮丝藻生长及产嗅味物质影响较小,强光照可促进2-MIB胞外释放,4000 lx时其胞外2-MIB分泌量达最高。小型饮用水供水湖库通过调节湖库蓄水量改变水温、引入水质较好的蓄水水源降低水库富营养程度,可避免蓝藻水华和嗅味物质污染事件的发生。Planktothrix sp.is a cyanobacteria species broadly distributed in water bodies across China and can produce massive blooms under favorable conditions.One of its secondary metabolites,2-methyl⁃isoborneol(2-MIB),dramatically decreases the sensory quality of drinking water because of its strong,undesirable musty odor.In this study,we investigated the effects of water temperature,nutrient concentra⁃tion(BG-11 media)and light intensity on the growth of Planktothrix sp.,and the production and extracel⁃lular secretion of 2-MIB.The Planktothrix sp.FACHB-1372 used in this study was isolated from Chao⁃hu Lake and cultured in medium to the logarithmic phase with a density of 3-5×106 cell/mL.Treatments included four temperatures(15,20,25,30℃),four BG-11 concentrations(20%,30%,50%,100%)and four illumination intensities(1000,2000,3000,4000 lx).All experiments lasted 14 days,and Plankto⁃thrix sp.samples were collected every 2 days for determination of cell density and 2-MIB content.Results show that,under the experimental conditions,temperature had the most largest effect on cell growth,2-MIB production and secretion.The optimal growth temperature of Planktothrix sp.was 25℃,yielding a cell density of 6.04×10^(5) cell/mL.Maximum 2-MIB production(3.64×10^(3)ng/L)and secretion(2.78×10^(3)ng/L)occurred at 30℃.At the lowest temperature(15℃)Planktothrix sp.grew slowly and 2-MIB production was not detected.Cell growth and total 2-MIB production increased with BG-11 concen⁃tration,but secretion of 2-MIB was higher at the low BG-11 concentration,an indication that low nutri⁃ent concentration promotes secretion of 2-MIB.Light intensity had the least effect on Planktothrix sp.growth,but growth did increase with illumination intensity from 1000-3000 lx.High illumination intensi⁃ty also promoted the release of 2-MIB,with secretion reaching the highest level at 4000 lx.Thus,to avoid and control Planktothrix sp.blooms and odor problems,we recommend regulating water storage in reservoirs to minimize water temperatur

关 键 词：浮丝藻 二-甲基异莰醇 温度 营养盐浓度 光照强度 

分 类 号：Q89[生物学]