Identification and functional characterization of ApisOr23 in pea aphid Acyrthosiphon pisum  

作　　者：HUANG Tian-yu ZHANG Rui-bin YANG Lu-lu CAO Song Frederic FRANCIS WANG Bing WANG Gui-rong 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [2]Guangdong Laboratory for Lingnan Modern Agriculture(Shenzhen Branch),Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China [3]Functional and Evolutionary Entomology,Gembloux Agro-Bio Tech,University of Liege,Gembloux 5030,Belgium

出　　处：《Journal of Integrative Agriculture》2022年第5期1414-1423,共10页农业科学学报（英文版）

基　　金：funded by the National Natural Science Foundation of China(31572072 and 31725023);the Intergovernmental International Science,Technology and Innovation Cooperation Key Project,China(2019YFE0105800);the Shenzhen Science and Technology Program,China(KQTD20180411143628272)。

摘　　要：Pea aphid,Acyrthosiphon pisum,is a serious pest of many different leguminous plants,and it mainly relies on its odorant receptors(Ors)to discriminate among host species.However,less is known about the role that Ors play in the host plant location.In this study,we identified a novel conserved odorant receptor clade by phylogenetic analysis,and conducted the functional analysis of ApisOr23 in A.pisum.The results showed that the homologous Ors from A.pisum,Aphis glycines and Aphis gossypii share 94.28% identity in amino acid sequences.Moreover,conserved motifs were analyzed using the annotated homologous Or23 from eight aphid species,providing further proof of the high conservation level of the Or23 clade.According to the tissue expression pattern analysis,ApisOr23 was mainly expressed in the antennae.Further functional study using a heterologous Xenopus expression system revealed that ApisOr23 was tuned to five plant volatiles,namely trans-2-hexen-1-al,cis-2-hexen-1-ol,1-heptanol,4’-ethylacetophenone,and hexyl acetate.Among them,trans-2-hexen-1-al,which is one of the main volatile organic compounds released from legume plants,activated the highest response of ApisOr23.Our findings suggest that the conserved Or23 clade in most aphid species might play an important role in host plant detection.

关 键 词：Acyrthosiphon pisum odorant receptor phylogenetic analysis two-electrode voltage clamp trans-2-hexen-1-al 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]