辣椒CaPIP5K基因家族的鉴定与特异性表达分析  

Identification and Specific Expression Analysis of CaPIP5K Gene Family in Pepper(Capsicum annuum)

作　　者：王永富 赵淑芳 苟秉调 段盼盼 魏敏 杨楠 张高原[1] 魏兵强[1] WANG Yong-Fu;ZHAO Shu-Fang;GOU Bing-Diao;DUAN Pan-Pan;WEI Min;YANG Nan;ZHANG Gao-Yuan;WEI Bing-Qiang(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,兰州730070

出　　处：《农业生物技术学报》2022年第4期641-655,共15页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31760572);甘肃农业大学青年导师扶持基金(GAU-QDFC-2020-07);甘肃省高校创新基金(2020B-128)。

摘　　要：磷脂酰肌醇信号系统在植物生长发育过程中起重要作用。磷酸脂酰肌醇-4-磷酸-5-激酶(phosphatidylinositol 4-phosphate 5-kinase,PIP5K)是该信号系统中的关键酶之一,能够催化磷脂酰肌醇-2-磷酸的合成。本研究在辣椒(Capsicum annuum)基因组数据库中共鉴定出19个CaPIP5K基因家族成员,其中14个CaPIP5Ks基因不均匀地定位于8条染色体上,其余5个CaPIP5Ks基因位于0号染色体上。所有CaPIP5K蛋白均含有PIP5K结构域,其中8个CaPIP5K蛋白还含有5~7个串联重复的MORN(membrane occupation and recognition nexus)结构域。亚细胞定位预测显示,所有CaPIP5K蛋白均定位于细胞质中。CaPIP5K基因家族成员的启动子顺式作用元件主要响应激素、防御与胁迫以及低温应答。基因表达结果表明,CaPIP5K4-1在花中的表达量远高于其他组织,并且在可育材料花蕾发育的最后阶段显著高于不育系,说明该基因不仅在花蕾中特异性表达,而且与辣椒雄性育性显著相关。本研究为进一步探讨CaPIP5K基因家族的功能及其在辣椒花粉与花药发育中的作用提供了参考依据。Phosphatidylinositol signaling system plays an important role in plant development.Phosphatidylinositol 4-phosphate 5-kinase(PIP5K)is one of the most crucial enzyme that catalyze PIP2 synthesis in this signaling system.The present study identified 19 CaPIP5K family genes in pepper(Capsicum annuum)genome database,among which 14 CaPIP5Ks were dispersedly distributed on 8 out of the 12 chromosomes and 5 CaPIP5Ks located on chromosome 0.Except 1 PIP5K domain was confirmed in every CaPIP5K proteins,5~8 repeated MORN(membrane occupation and recognition nexus)domains were also detected in 8 CaPIP5Ks.All CaPIP5K proteins were predicted to locate in the cytoplasm.Moreover,cis-acting elements in CaPIP5K promoter region mainly responded to hormones,defense and stress,and low temperature.In addition,the expressional analysis indicated that CaPIP5K4-1 not only specifically expressed in flower instead of other tissues,but also gradually improved with the development of flower buds in fertile accessions as compared to sterile accession.This study provides a reference basis for further exploring the mechanism of CaPIP5K gene family in the development of anther and pollen in pepper.

关 键 词：辣椒 磷酸脂酰肌醇-4-磷酸-5-激酶(PIP5K) 基因家族 雄性育性 表达分析 

分 类 号：S641.3[农业科学—蔬菜学]