基于SLAF的甜瓜SNP遗传连锁图谱构建和种子性状QTL定位  

Construction of SNP Genetic Linkage Map and QTL Mapping of Seed Traits in Melon(Cucumis melo)Based on SLAF

作　　者：李俊凤 王岭[1] 戴冬洋 才羿 杨丽敏 王超[1] 盛云燕[1] 田丽美[1] LI Jun-Feng;WANG Ling;DAI Dong-Yang;CAI Yi;YANG Li-Min;WANG Chao;SHENG Yun-Yan;TIAN Li-Mei(College of Horticulture and Landscape Architecture,Heilongjiang Bayi Agricultural Reclamation University,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学园艺园林学院,大庆163319

出　　处：《农业生物技术学报》2022年第4期656-666,共11页Journal of Agricultural Biotechnology

基　　金：黑龙江省应用技术研究与开发计划项目(GA19B102);黑龙江八一农垦大学三横三纵支持计划(TDJH202004)。

摘　　要：种子相关性状是作物重要的农艺性状,对甜瓜(Cucumis melo)遗传育种及其相关分子生物学研究具有重要意义。本研究选择薄皮甜瓜小粒种子品系'P5'为母本、厚皮甜瓜大粒种子品系'P10'为父本,配置杂交组合,获得F1、F2群体,利用450个F2单株开展种子相关性状遗传分析,结果显示,种子形状和种皮颜色符合3∶1的分离比例(χ^(2)=0.26和0.07),两者均为1对等位基因控制的质量性状;种子长度、种子宽度和种子百粒重均呈单峰正态分布,为典型的数量性状。利用127个F2单株开展特异性位点扩增片段测序(specific-locus amplified fragment sequencing,SLAF-seq)并构建高饱和甜瓜遗传图谱,对种子相关性状开展QTL初步分析。构建的甜瓜遗传图谱包含12个连锁群,获得了3716个上图标记,总图距为1356.49 cM,标记间平均遗传距离为0.37 cM;将控制甜瓜种子形状(seed shape,SS)的位点SS3.1定位在甜瓜3号染色体31470843~31659961 bp,表型贡献率为26.45%;将控制甜瓜种皮颜色(seed color,SC)的位点SC12.1定位在甜瓜12号染色体12785226~15029452 bp,表型贡献率为14.07%;检测到控制甜瓜种子百粒重(hundred of seed weight,HSW)的2个QTL位点(HSW6.1和HSW7.1),HSW6.1位于甜瓜6号染色体1082887~1206028 bp,表型贡献率为13.97%,HSW7.1位于甜瓜7号染色体458518~1212963 bp,表型贡献率为9.76%;检测到1个控制甜瓜种子长度(seed length,SL)的QTL位点(SL3.1),位于甜瓜3号染色体31430517~31553644 bp,表型贡献率为27.43%;检测到2个控制甜瓜种子宽度(seed width,SW)的QTL位点(SW3.1和SW12.1),SW3.1位于甜瓜3号染色体31447562~31470545 bp,表型贡献率为16.03%,SW12.1位于甜瓜12号染色体1254239~1313909 bp,表型贡献率为12.88%。研究结果为进一步克隆甜瓜种子相关性状的基因挖掘与功能分析提供理论依据。Seed-related traits are important agronomic traits of crops,which are of great significance to melon(Cucumis melo)genetics and breeding and related molecular biology research.In this study,the thin-skinned melon small seed line'P5'was used as the female parent,and the thick-skinned melon big seed line'P10'was used as the male parent,F_(1) and F_(2) populations were obtained,450 F_(2) plants were used to carry out genetic analysis of seed related traits.The results showed that the segregation ratio of seed shape and seed coat color was consistent with 3∶1(χ^(2)=0.26,0.07).Both of them were quality traits controlled by a pair of alleles.The results of F_(2) population distribution showed that seed length,seed width and 100-seed weight showed unimodal normal distribution,which were quantitative traits.The 127 F2 individual plants were used to carry out specific-locus amplified fragment sequencing(SLAF-seq)technique and construct a genetic map of high saturation melon,and preliminary QTL analysis of seed-related characters was carried out.The results showed that a melon genetic map containing 12 linkage groups was constructed,and 3716 markers were obtained,the total map distance was 1356.49 cM,and the average genetic distance between markers was 0.37 cM.The locus SS3.1 controlling melon seed shape(SS)was located between Marker555072 and Marker555263 on melon chromosome 3,and the distance from the linkage markers on both sides was 83.91 and 84.34 cM,respectively,with a contribution rate of 26.45%.The locus SC12.1 controlling melon seed color(SC)was located between Marker1467315 and Marker1479935 on melon chromosome 12,and the distance from the linkage markers on both sides was 62.60 and 63.44 cM,respectively,and the contribution rate was 14.07%.Two QTL loci(HSW6.1 and HSW7.1)were detected to control the 100-seed weight of muskmelon seeds.HSW6.1 is located between Marker795158 and Marker795692 on chromosome 6,and the distances from the linkage markers on both sides are 17.67 and 21.03 cM,respectively,with a contribu

关 键 词：甜瓜 特异性位点扩增片段(SLAF)遗传图谱 种子相关性状 QTL 

分 类 号：S652[农业科学—果树学]