济宁百日鸡pri-miR-458b基因多态性及其与产蛋性状的关联分析  

Polymorphisms of pri-miR-458b Gene and Its Association Analysis with Laying Performance in Jining Bairi Chickens(Gallus gallus)

作　　者：韩旭 宋志方 滕军 沈国蕊 武文惠 王文文[1] 唐辉[1] HAN Xu;SONG Zhi-Fang;TENG Jun;SHEN Guo-Rui;WU Wen-Hui;WANG Wen-Wen;TANG Hui(College of Animal Science and Technology,Shandong Agricultural University,Tai'an 271018,China)

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《农业生物技术学报》2022年第4期704-715,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31872344)。

摘　　要：miR-458b-5p介导Wnt/β-连环蛋白(β-catenin)信号通路来调节鸡(Gallus gallus)卵巢卵泡发育,从而影响家禽产蛋水平。pri-miR-458b的SNP影响miR-458b的加工过程。为探究pri-miR-458b基因多态性与产蛋性状的关系,本研究通过直接测序法检测了pri-miR-458b基因的SNP,并将检测到的SNP与济宁百日鸡的产蛋性状进行关联分析。结果表明,pri-miR-458b基因在鸡群中共检测出30个SNP,有10个位点与产蛋性状相关,其中的rs316393825、rs734005133、rs737338503、rs733344248、rs740622221和rs7337777096个位点连锁不平衡,其联合基因型对42~49周产蛋数和产蛋总数有显著影响;单倍型AGCGCG可使42~49周产蛋数增加3.29枚,产蛋总数增加8.08枚。相较于AGCGCG型的自由能,GGCGCG型的自由能降低了3.3 kcal/mol,而GTTAAA型的自由能升高了5.3 kcal/mol。GGCGCG型的二级结构稳定性最高,AGCGCG型次之,GTTAAA型最低。本研究可为济宁百日鸡产蛋性状辅助选择提供有利用价值的分子遗传标记。miR-458b-5p mediates the Wnt/β-catenin signaling pathway to regulates the development of chicken(Gallus gallus)ovarian follicles,thus affecting the level of laying in poultry.The SNP of pri-miR-458b affects the processing of miR-458b.To explore the association of pri-miR-458b gene polymorphisms with laying trait,in this study,SNPs in pri-miR-458b gene were detected with the direct sequencing and then their correlations with the laying traits were analyzed in Jining Bairi chickens.The results showed that a total of 30 SNPs were detected in the pri-miR-458b gene,and 10 loci of them were related to laying traits.Among them,rs316393825,rs734005133,rs737338503,rs733344248,rs740622221 and rs733777709 were linkage imbalance.Their combined genotype significantly affected egg number from 42 to 49 week of age and egg number until 49 week of age.The dominant haplotype of AGCGCG could increase egg number from 42 to 49 week of age by 3.29 and egg number until 49 week of age by 8.08.Compared with the free energy of AGCGCG type,the free energy of GGCGCG type decreased by 3.3 kcal/mol,while that of GTTAAA type increased by 5.3 kcal/mol.The secondary structure stability of GGCGCG type was the highest,AGCGCG type was the second,and GTTAAA type was the lowest.This study provides valuable molecular genetic markers for auxiliary selection of laying traits in Jining Bairi chickens.

关 键 词：济宁百日鸡 pri-miR-458b SNP 产蛋性状 关联分析 

分 类 号：S831.2[农业科学—畜牧学]