灰葡萄孢bZIP家族基因的全基因组鉴定与表达规律分析  

Genome-wide Identification and Expression Analysis of bZIP Family Genes in Botrytis cinerea

作　　者：李白 张子妍 张强 曹宏哲 藏金萍[1,2] 张康 邢继红 董金皋[2,3,4] LI Bai;ZHANG Zi-Yan;ZHANG Qiang;CAO Hong-Zhe;ZANG Jin-Ping;ZHANG Kang;XING Ji-Hong;DONG Jin-Gao(College of Life Sciences,Hebei Agricultural University,Baoding 071000,China;Hebei Key Laboratory of Plant Physiology and Molecular Pathology,Baoding 071000,China;State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071000,China;College of Plant Protection,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学生命科学学院,保定071000 [2]河北省植物生理与分子病理学重点实验室,保定071000 [3]省部共建华北作物改良与调控国家重点实验室,保定071000 [4]河北农业大学植物保护学院,保定071000

出　　处：《农业生物技术学报》2022年第4期762-771,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31972217,32072369);中央引导地方科技发展基金(206Z6501G)。

摘　　要：bZIP(basic leucine zipper,bZIP)转录因子广泛存在于植物病原真菌中,在病菌的形态建成和侵染过程中起着重要的调控作用。灰葡萄孢(Botrytis cinerea)bZIP转录因子家族的系统研究少有报道。本研究利用生物信息学方法,在全基因组水平上对灰葡萄孢bZIP家族基因进行鉴定,并对其蛋白理化性质、系统进化关系、保守结构域及其在病菌分生孢子发育时期和侵染时期的表达规律进行分析。同时,利用qPCR技术,检测灰葡萄孢bZIP家族基因在NaCl、H2O2处理后的表达情况。结果发现,灰葡萄孢基因组中共包含16个bZIP家族基因,系统进化分析将其分为4个亚家族;灰葡萄孢bZIP家族基因均含有bZIP家族典型的BRLZ结构域,部分基因在分生孢子的不同发育和侵染时期上调表达,在NaCl和H2O2处理后显著下调表达,表明灰葡萄孢bZIP家族基因在病菌生长发育、侵染过程以及响应盐胁迫和氧化胁迫方面发挥重要的作用,本研究为阐明灰葡萄孢bZIP家族基因的功能及其作用机制提供了理论依据。The bZIP(basic leucine zipper,bZIP)transcription factors are widely present in phytopathogenic fungi,and play an important regulatory role in the morphogenesis and infection of pathogens.Systematic studies of the bZIP transcription factor family in Botrytis cinerea have been rarely reported.In this study,the bZIP family genes in Botrytis cinerea were genome-wide identified,the protein physicochemical properties,phylogenetic evolution,conserved domain,and the expression pattern in pathogen conidia during development and infection period were analyzed.Meanwhile,qPCR was used to detect the expression of bZIP family genes after NaCl and H_(2)O^(2) treatment.The results showed that there were 16 bZIP family genes identified from Botrytis cinerea genome,which were divided into 4 subfamilies by phylogenetic analysis.All bZIP family genes of Botrytis cinerea contained the typical BRLZ domain of bZIP family.Some genes showed high expression levels at different development of conidia and infection stages of Botrytis cinerea.All bZIP family genes of Botrytis cinerea were significantly down-regulated after NaCl and H_(2)O_(2) treatment.These results indicated that bZIP family genes of Botrytis cinerea played an important role in the growth and development,infection process and response to salt stress and oxidative stress.This study provides a theoretical basis for further revealing the function and molecular mechanism of bZIP family genes of Botrytis cinerea.

关 键 词：灰葡萄孢 bZIP家族基因 生物信息学分析 表达分析 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]