动物毛干纤维物种来源的快速分子鉴定  

A Quick Molecular Identification for the Species Sources of Animal Fibres

作　　者：向海 朱飘 张兴 杨柳 赵兴波[1,2] XIANG Hai;ZHU Piao;ZHANG Xing;YANG Liu;ZHAO Xing-Bo(Guangdong Provincial Key Laboratory of Animal Molecular Design and Precision Breeding,College of Life Science and Engineering,Foshan University,Foshan 528231,China;College of Animal Science and Technology,China Agricultural University,Beijing 100193,China)

机构地区：[1]佛山科学技术学院生命科学与工程学院/广东省动物分子设计与精准育种重点实验室,佛山528231 [2]中国农业大学动物科技学院,北京100193

出　　处：《农业生物技术学报》2022年第4期802-808,共7页Journal of Agricultural Biotechnology

基　　金：广东省普通高校青年创新人才项目(2019KQNCX167);国家自然科学基金(31961133031);佛山科学技术学院高层次人才科研启动项目(CGG07154)。

摘　　要：动物毛干纤维物种来源鉴定是纺织品检测工作的重要内容之一,具有较大的技术需求。本研究利用物种间线粒体DNA特异性,设计毛绒制品常见动物源包括牦牛(Bos grunniens)、单峰驼(Camelus dromedarius)、双峰驼(Camelus bactrianus)、绵羊(Ovis aries)、山羊(Capra hircus)、普通牛(Bos taurus)及家猪(Sus scrofa)等多个物种的通用引物,同时设计牦牛、骆驼、绵羊、山羊的特异性引物以及绵羊、山羊通用引物。通过对比动物纤维DNA提取方法,建立了从动物毛干中高效提取DNA的实验流程,利用设计的多物种通用引物扩增测序,实现对样本保存状况及混杂程度等进行准确、快速判定,利用设计的各物种特异引物扩增电泳检测,进一步实现快速、低成本的鉴别动物毛干纤维的物种来源。本研究建立了一种准确、快速、简便区别动物毛干纤维物种来源的分子鉴定体系,为动物纺织产品的质量控制提供了技术参考。Identification of species origin of animal fibres is important in textile inspection work.In this study,a universal primer set for yak(Bos grunniens),camel(Camelus dromedarius and Camelus bactrianus),sheep(Ovis aries),goat(Capra hircus),cattle(Bos taurus)and pig(Sus scrofa)were designed according to their mitochondrial genomes.Moreover,species-specific primer sets for yak,camel,sheep and goat as well as a universal primer set for goat and sheep were also generated.After that,several DNA extraction methods were compared to find out the most optimal method for animal wool shaft fiber.Then the preservation status of samples were determined rapidly and examined whether it was mixed accurately by sequencing the amplicon of the universal primer set.By combining the species-specific primers amplification and electrophoresis detection,we are able to identify the animal species sources of wool shaft fibers with limit time and cost.In this study,an accurate,rapid and simple molecular identification system was established to distinguish the animal species sources of wool shaft fibers,which provides a technical reference for the quality control of textile products.

关 键 词：毛干纤维 分子鉴定 物种来源 PCR扩增 Sanger测序 

分 类 号：S872[农业科学—畜牧兽医]