机采献血者隐匿性HBV感染与HLA-DP基因变异的相关性研究  

Study on the relationship between occult HBV infection and HLA-DP gene variation in platelet donors

作　　者：竺青[1] 周娟[1] 孙桂芝[1] 刘丽丽 赵卓[2] 陈元锋[1] ZHU Qing;ZHOU Juan;SUN Gui-zhi;LIU Li-li;ZHAO Zhuo;CHEN Yuan-feng(Shandong Hematology Center,Jinan,Shandong 250014;Department of Cardiology,Jinan Central Hospital,Jinan,Shandong 250013,China)

机构地区：[1]山东省血液中心机采科,山东济南250014 [2]济南市中心医院心内科,山东济南250013

出　　处：《热带医学杂志》2022年第2期145-148,153,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(81670245)。

摘　　要：目的研究机采献血者隐匿性乙型肝炎病毒(HBV)感染(OBI)与人类白细胞抗原-DP(HLA-DP)基因变异的相关性。方法选择2018年1月-2019年12月山东省血液中心成功捐献血小板、乙肝表面抗原(HBsAg)阴性、NAT检测无反应的献血者1346例为研究对象,提取献血者血液DNA,采用直接测序法进行HLA-DP基因分型,cobas HBV定量核酸检测试剂盒检测血液HBV-DNA载量,Logistic回归分析HLA-DP基因变异与OBI的关系。结果OBI与非OBI HLA-DPB1 rs3135021等位基因频率及基因型频率比较差异均无统计学意义(P>0.05);与非OBI比较,OBI献血者HLA-DPA1 rs3077位点T等位基因频率、TT基因型频率显著增加,C等位基因频率、CC基因型频率显著降低,差异有统计学意义(P<0.05);HLA-DPB1 rs9277535位点A等位基因频率、GA等位基因型频率显著增加,G等位基因频率、GG基因型频率显著降低,差异有统计学意义(P<0.05)。OBI献血者HLA-DPA1 rs3077位点、HLA-DPB1rs9277535位点多态性与HBV-DNA载量有关,差异有统计学意义(P<0.05)。Logistic回归分析结果显示,HLA-DPA1rs3077位点携带TT基因型、HLA-DPB1 rs9277535位点携带GA、AA基因型是影响OBI发生的独立危险因素(P<0.05)。结论机采献血者中OBI可能与HLA-DPA1 rs3077位点、HLA-DPB1 rs9277535位点多态性有关。Objective To study the relationship between occult hepatitis B virus(HBV) infection(OBI) and human leukocyte antigen-DP(HLA-DP)gene variation in platelet donors.Methods From January 2018 to December 2019,1 346 blood donors who successfully donated blood platelets and had no response to hepatitis B surface antigen(HBsAg)(-),NAT test in our blood center were selected as the research objects.DNA was extracted from blood donors.HLA-DP genotyping was performed by direct sequencing;cobas HBV quantitative nucleic acid detection kit was used to detect HBV-DNA load in blood,and Logistic regression was used to analyze the relationship between HLA-DP gene variation and OBI.Results There was no significant difference in allele frequency and genotype frequency of HLA-DPB1 rs3135021 between OBI and non-OBI(P>0.05).Compared with non-OBI,the frequencies of T allele and TT genotype at HLA-DPA1 rs3077 locus in OBI donors increased significantly,while the frequencies of C allele and CC genotype decreased significantly(P<0.05);the frequencies of A allele and GA allele at HLA-DPB1 rs9277535 locus increased significantly,while the frequencies of G allele and GG genotype decreased significantly(P<0.05).The polymorphisms of HLA-DPA1 rs3077 and HLA-DPB1 rs9277535 were related to HBV-DNA load(P<0.05).Logistic regression analysis showed that TT genotype at HLA-DPA1 rs3077 locus and GA and AA genotype at HLA-DPB1 rs9277535 locus were independent risk factors for OBI(P<0.05).Conclusion OBI in platelet donors might be related to the polymorphisms of HLA-DPA1 rs3077 locus and HLA-DPB1 rs9277535 locus.

关 键 词：机采献血者 隐匿性乙型肝炎病毒感染 人类白细胞抗原-DP 基因变异 

分 类 号：R512.62[医药卫生—内科学]