褪黑素对控制性超排卵周期中体外成熟卵母细胞受精后所获胚胎发育的影响  

Effect of melatonin on embryo development after fertilization of in vitro matured oocytes in controlled ovarian hyperstimulation cycles

作　　者：朱琦 丁丁[2] 王凯娟 霍然[3] 张学森[3] 刘婷婷 曹云霞[2] 章志国[1,2] Zhu Qi;Ding Ding;Wang Kaijuan;Huo Ran;Zhang Xuesen;Liu Tingting;Cao Yunxia;Zhang Zhiguo(Dept of Biomedical Engineering,Anhui Medical University,Hefei 230032;Dept of Obstetrics and Gynecology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;State Key Laboratory of Reproductive Medicine,Nanjing Medical University,Nanjing 211166;Medical Record Information Office of Anhui Thoracic Hospital,Hefei 230022)

机构地区：[1]安徽医科大学生物医学工程学院,合肥230032 [2]安徽医科大学第一附属医院妇产科,合肥230022 [3]南京医科大学生殖医学国家重点实验室,南京211166 [4]安徽省胸科医院病案信息室,合肥230022

出　　处：《安徽医科大学学报》2022年第4期616-621,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82071724);安徽高校自然科学研究项目(编号:KJ2020A0202);生殖医学国家重点实验室开放课题(编号:SKLMR-K202005)。

摘　　要：目的探究不同浓度褪黑素(MT)对控制性超排卵(COH)周期中体外成熟卵母细胞受精后所获胚胎发育的影响。方法收集COH周期的人未成熟卵母细胞行体外成熟(IVM)培养及单精子胞浆内注射(ICSI)受精,接着依次用含0、10^(-11)、10^(-9)、10^(-7)及10^(-5)mol/L MT的培养液进行分裂期和囊胚期胚胎体外培养,收集和冻存优质囊胚,最后通过array-CGH对复苏后优质囊胚进行非整倍体检测。结果10^(-9)mol/L组的囊胚率高于10^(-11)、10^(-7)、10^(-5)及0 mol/L组,差异有统计学意义(P<0.05,P<0.01,P<0.0001,P<0.01);10^(-9)mol/L组的优质囊胚率均高于其他组,但仅与10^(-5)mol/L和0 mol/L组相比,差异有统计学意义(P<0.01,P<0.05);MT组的非整倍体发生率(17.6%)低于非MT组(33.3%),但差异无统计学意义。结论人类胚胎培养液中添加MT可以促进胚胎的体外发育,这种促进作用存在浓度相关性,10^(-9)mol/L是最佳的作用浓度。Objective To explore the effects of melatonin(MT)on the fertilization of in vitro matured oocytes in controlled ovarian hyperstimulation(COH)cycles and the development of subsequent embryos.Methods Imma-ture human oocytes from COH cycles were collected for in vitro maturation(IVM)culture and intracytoplasmic sperm injection(ICSI)insemination,and then the cleavage embryo and blastocyst culture medium supplemented with 0,10^(-11),10^(-9),10^(-7)or 10^(-5)mol/L MT were used in order to perform embryo culture in vitro,next the formed high-quality blastocysts were picked up and cryopreserved via vitrification,finally,array-CGH technology was used to detect aneuploidy of rewarmed high-quality blastocysts.Results The blastocyst rate of 10^(-9) mol/L group was significantly higher than that of 10^(-11),10^(-7),10^(-5)and 0 mol/L groups(P<0.05,P<0.01,P<0.0001,P<0.01),respectively;the high-quality blastocyst rate of 10^(-9) mol/L group was higher than that of other groups,but there were only significant differences compared with 10^(-5)mol/L and 0 mol/L groups(P<0.01,P<0.05);the incidence of aneuploidy in MT group(17.6%)was lower than that in non-MT group(33.3%),but no significant difference was found.Conclusion The addition of MT to human embryo culture medium can promote the in vitro development of embryo,which is related to concentration,and 10^(-9)mol/L is the optimal concentration.

关 键 词：褪黑素 胚胎体外培养 胚胎发育 非整倍体 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R329[医药卫生—基础医学]