HPLC法测定不同产地栀子提取物的含量  

Determination of the content of Gardenia jasminoides Ellis from different producing areas by HPLC

作　　者：武文涛 赵育林 刘亚智 Wentao Wu;Yulin Zhao;Yazhi Liu(Medical College of Zhangjiakou University,Zhangjiakou,Hebei 075000,China)

机构地区：[1]张家口学院医学院,河北张家口075000

出　　处：《日用化学工业》2022年第4期451-456,共6页China Surfactant Detergent & Cosmetics

摘　　要：通过高效液相色谱法(HPLC)测定不同产地栀子中主要成分栀子苷、栀子黄色素等含量,对不同产地的栀子进行比较。采用C_(18)(4.6 mm×250 mm,5μm)色谱柱,水相为0.2%磷酸溶液,有机相为甲醇-乙腈,检测波长为254 nm,洗脱速度为1 mL/min,在柱温30℃条件下进行不同产地栀子中主要成分的测定。实验结果表明,供试品溶液的10种主要成分线性关系良好,平均加样回收率为97.3%~103.4%,通过比较不同产地栀子提取物含量的测定结果可知药材产地为江西平远县的栀子提取物中主要成分含量最高。The contents of the main components of Gardenia,such as geniposide and gardenia yellow pigment,were determined by HPLC method,and the gardenia from different areas were compared.By using a C_(18)(4.6 mm×250 mm,5μm)chromatographic column,the aqueous phase uses 0.2%phosphoric acid solution,the organic phase uses methanolacetonitrile,the detection wavelength is 254 nm,the elution rate is 1 mL/min,and the column temperature is 30℃.The determination of the main components in Gardenia from different origins was carried out below.The experimental results show that the 10 main components of the solution have a good linear relationship,and the average sample recovery rate is 97.3%-103.4%.By comparing the determination results of Gardenia extracts from different origins,it can be seen that the Gardenia extract from Pingyuan County,Jiangxi Province has the highest amount of the main contents.The experimental method used in this paper has certain reliability and can help the medicinal research of Gardenia.

关 键 词：栀子提取物 高效液相色谱法 有效成分 机制研究 

分 类 号：TQ658[化学工程—精细化工]