中国南海软珊瑚共附生真菌Emericellopsis sp.SCSIO41202的次级代谢产物研究  

Research about the secondary metabolites of soft coral-derivedepiphytic fungus Emericellopsis sp.SCSIO41202 from the South China Sea

作　　者：邵苏润 杨斌[1] 李慧敏 付春钰 刘永宏 李云秋[1] SHAO Surun;YANG Bin;LI Huimin;FU Chunyu;LIU Yonghong;LI Yunqiu(Guilin Medical University,Guilin 541199,China)

机构地区：[1]桂林医学院,广西桂林541199

出　　处：《华夏医学》2022年第1期7-12,共6页Acta Medicinae Sinica

摘　　要：目的:探索和挖掘中国南海软珊瑚来源的共附生真菌Emericellopsis sp.SCSIO41202次级代谢产物中结构新颖的化合物。方法:采用TLC、MPLC和半制备柱HPLC法分离纯化并运用NMR、MS以及与相关文献数据比对方法进行结构鉴定。结果:得到了已知单体化合物8个,既大豆苷元(1);5-甲氧基大豆苷元(2);染料木素(3);(3R,4S)-6、8-二羟基-3、4-二氢-3、4、5、7-四甲基异香豆素(4);(3R,4R)-4-乙酰基-3、4-二氢-6、8-二羟基-3-甲氧基-5-甲基异香豆素(5);(3S,4S)-4-乙酰基-3、4-二氢-6、8-二羟基-3-甲氧基-5-甲基异香豆素(6);烟曲霉酸(7);1H-吲哚-3-甲醛(8)。结论:化合物1、3、6、7、8对AChE和PL有抑制作用,其中化合物7的抑制作用较强,抑制率为41.7%。Objective:To explore and excavate novel compound from the secondary metabolites of Emericellopsis sp.SCSIO41202 isolated from the soft cora in South China Sea.Methods:The liquid extract were isolated by comprehensive chromatography methods including thin layer chromatography(TLC),positive or negative phase medium pressure preparative liquid chromatography(MPLC),and semipreparative high performance liquid chromatography(HPLC),and their structures were elaborated by magnetic resonance spectroscopy(NMR),mass spectrum(MS)and comparison with the physical and chemical properties and reported literatures.Results:Eight known compounds were acquired and discerned,which were daidzein(1);methoxydaidzein(2);genistein(3);(3R,4S)-6,8-dihydroxy-3,4-dihydro-3,4,5,7-tetramethylisocoumarin(4);(3R,4R)-4-acetyl-3,4-dihydro-6,8-dihydroxy-3-methoxy-5-methylisocoumarin(5);(3S,4S)-4-acetyl-3,4-dihydro-6,8-dihydroxy-3-methoxy-5-methylisocoumarin(6);helvolic acid(7);1 H-indole-3-formaldehyde(8).Conclusion:Compounds 1,3,6,7 and 8 had inhibitory effects on ache and PL,among which compound 7 had strong inhibitory effect,and the inhibition rate was 41.7%.

关 键 词：Emericellopsis sp. 软珊瑚 次级代谢产物 

分 类 号：R915[医药卫生—微生物与生化药学]