单细胞转录组测序分析揭示腹膜透析患者透析流出液T淋巴细胞的改变  

Single-cell transcriptome analysis reveals changes of T lymphocytes in peritoneal fluid from peritoneal dialysis patients

作　　者：李咏倢 孙宇翔 林洪春 李灿明[1] 尚红利[1] 彭晖[1] Li Yongjie;Sun Yuxiang;Lin Hongchun;Li Canming;Shang Hongli;Peng Hui(Department of Nephrology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China)

机构地区：[1]中山大学附属第三医院肾内科,广州510630

出　　处：《新医学》2022年第4期292-296,共5页Journal of New Medicine

基　　金：国家自然科学基金(81873613)。

摘　　要：目的 揭示腹膜透析(PD)患者透析流出液中T淋巴细胞随PD时间延长的改变轨迹,探究其与PD相关腹膜纤维化的关系。方法 对笔者前期研究获取的10例PD患者(短期PD组6例,长期PD组4例)透析流出液细胞的单细胞转录组数据进行细胞分类,提取出T淋巴细胞的数据进行2组间的差异分析、通路富集分析;同时收集23例患者PD治疗前及开始后随访的血常规数据,比较PD开始前后免疫细胞组成的差异。结果 PD患者透析流出液中T淋巴细胞在免疫细胞总数中的占比随PD时间延长增加(P <0.05)。差异分析显示转化生长因子-β1(TGF-β1)和其他纤维化相关的信号通路在长期PD组的腹腔T淋巴细胞中富集。与开始透析前全血细胞计数结果比较,短期及长期PD组患者的淋巴细胞计数均升高,但在比例上呈现为先上升后下降的趋势。结论 长期PD治疗导致的腹膜纤维化与T淋巴细胞密切相关,PD流出液中T淋巴细胞的变化可能是腹腔环境等多因素共同作用的结果。Objective To reveal the change trajectory of T lymphocytes in the dialysis effluent from peritoneal dialysis(PD) patients with the prolongation of PD time, and to explore its relationship with PD-associated peritoneal fibrosis. Methods Cell classification was performed on the single-cell transcriptome data of PD effluent cells from 10 PD patients(6 cases in the short-term PD group and 4 cases in the long-term PD group) obtained in our previous study, and the data of T lymphocytes were extracted to analyze the differences between two groups, and pathway enrichment analysis was also performed. At the same time, complete blood count data of 23 PD patients before and after PD were collected, and the differences in immune cell composition were compared before and after PD. Results The proportion of T lymphocytes to the total number of immune cells in the PD effluent was increased significantly with the prolongation of PD time(P < 0.05). Differential analysis showed that TGF-β1 and other fibrosisrelated signaling pathways were enriched in peritoneal T lymphocytes in the long-term PD group. Compared with the complete blood count before dialysis, the lymphocyte count in both short-term and long-term PD groups was significantly increased, but the proportion was initially increased, and subsequently decreased. Conclusions Peritoneal fibrosis caused by long-term PD treatment is closely associated with T lymphocytes. And the changes of T lymphocytes in PD effluent may be jointly caused by the peritoneal environment and alternative multiple factors.

关 键 词：单细胞转录组测序 腹膜透析 腹膜纤维化 T淋巴细胞 

分 类 号：R692.5[医药卫生—泌尿科学]