小蓟提取物抑制膀胱癌细胞T24的侵袭和迁移能力  

Cirsii herba extract inhibits invasion and migration of T24 bladder cancer cell

作　　者：林海青[1] 宋钦兰[1] 孙中华 曲远均[1] 李静蔚[1] 刘志勇 LIN Hai-qing;SONG Qin-lan;SUN Zhong-hua;QU Yuan-jun;LI Jing-wei;LIU Zhi-yong(Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Ji'nan Shandong 250014,China)

机构地区：[1]山东中医药大学附属医院,山东济南250014

出　　处：《时珍国医国药》2022年第2期305-308,共4页Lishizhen Medicine and Materia Medica Research

基　　金：山东省自然科学基金青年项目(ZR2020QH337);国家自然科学基金(81573989,81403408)。

摘　　要：目的探讨小蓟提取物对膀胱癌细胞侵袭和迁移的抑制作用。方法制备小蓟的水煎液(XJ-ST)、水提醇沉液(XJ-STCC)和醇提水沉液(XJ-CTSC),利用CCK-8试剂盒分别检测对T24细胞活力的影响,选取抑制效果最明显的XJ-CTSC,利用划痕实验检测对迁移的抑制作用,Transwell实验检测对侵袭的抑制作用,western blot检测MMPs和EMT相关蛋白表达水平变化。结果3种不同工艺的小蓟提取物中XJ-CTSC对T24细胞活力的抑制效果最为明显,并能显著抑制T24细胞的侵袭和迁移能力,上调CDH1的蛋白水平,下调CDH2、MMP2和MMP9的表达。结论XJ-CTSC能够有效抑制T24细胞的侵袭和迁移能力,是抗肿瘤转移药物开发的潜在候选者。Objective To investigate the inhibitory effect of extract from Cirsii herba on invasion and migration of bladder cancer cells.Methods The water decoction(XJ-ST),water extraction and alcohol precipitation(XJ-STCC)and alcohol extraction and water precipitation(XJ-CTSC)of Cirsii herba were prepared.CCK-8 kit was used to assess the effect of extractions on the viability of T24 cells.XJ-CTSC with the most obvious inhibitory effect was selected for further study.The inhibition on migration was detected by scratch assay,the inhibition on invasion was assayed by transwell test,and the expression levels of MMPs and EMT related proteins were evaluated by western blot.Results XJ-CTSC had the most obvious inhibitory effect on T24 cells,it can also significantly inhibit the invasion and migration of T24 cells,up regulate the protein level of CDH1 and down regulate the expression of CDH2,MMP2 and MMP9.Conclusion XJ-CTSC can effectively inhibit the invasion and migration of T24 cells,and is a potential candidate for the development of anti-metastasis drugs.

关 键 词：小蓟 膀胱癌 侵袭 迁移 

分 类 号：R285.5[医药卫生—中药学]