不同制备方法对青木香煎提物中马兜铃酸Ⅰ的转移研究  

Researches on the transfers of aristolochic acidⅠin the decocting extracts from Radix Aristolochiae using different methods

作　　者：罗子龙 李菌芳 赖珊 袁干军[1,2] 易文芳 孙一斐 LUO Zi-long;LI Jun-fang;LAI Shan;YUAN Gan-jun;YI Wen-fang;SUN Yi-fei(Biotechnological Engineering Center for Pharmaceutical Research and Development,Jiangxi Agricultural University,Nanchang 330045,China;College of Bioscience and Bioengineering,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学生物技术药物研发工程中心,江西南昌330045 [2]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《时珍国医国药》2022年第2期370-372,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金地区基金(81660578,81460529)。

摘　　要：目的考察不同制备方法对青木香煎煮提取物中马兜铃酸I的转移情况,为含马兜铃酸类毒性成分中药的安全用药提供参考。方法从模拟家庭的煎药方式、企业的中药煎煮提取和一般实验室煎煮的实际出发,采用高效液相色谱法,对青木香采用不同器皿煎提(陶瓷砂锅、不锈钢锅或玻璃锥形瓶)、干燥方法(冷冻干燥、敞口式浓缩或减压浓缩)和煎药方式(单煎、复方煎煮或配方颗粒的分煎)所得煎提物中马兜铃酸I的含量进行测定。结果青木香采用不锈钢锅或砂锅煎煮、敞口浓缩、复方配伍煎煮时,马兜铃酸I的转移明显少于玻璃锥形瓶煎煮、冷冻干燥或减压浓缩干燥、单煎或配方颗粒的制药方式。结论青木香等含马兜铃酸类化合物的中药宜采用陶瓷砂锅或不锈钢材质的设备煎提、生产时优先敞口浓缩收膏干燥、且煎提时宜复方配伍共煎,不宜单煎或以配方颗粒的形式制药,由此为含马兜铃酸等毒性成分中药的安全用药提供参考与借鉴。Objective To examine the transfer of aristolochic acid I in decoction extracts of Radix aristolochiae by different preparation methods,and to provide references for the safe use of chinese medicines containing aristolochic acids.Methods According to the actual situation of family decoction,decoctions of chinese medicines in production scale and in general laboratory scale,the contents of aristolochic acid I in the decoction extracts,obtained with different containers(ceramic casserole,stainless steel pot and glass conical flask),drying methods(freeze-drying,open concentration and reduced pressure concentration),and decoction methods(separated decoction,recipe granular decoction and mixed decoction),of Radix aristolochiae were determined using high performance liquid chromatography using.Results The transfer of aristolochic acid I from Radix aristolochiae decocted using stainless steel or casserole,concentrated under exposure status,and decocted in mixed prescription was significantly less than that decocted using glass conical flask,freeze-drying or concentrated under decompression condition,decocted in separation or in single recipe.Conclusion It is better for Radix aristolochiae,together with other chinese medicines containing aristolochic acids,to be decocted using ceramic casserole or equipment prepared with stainless steel.In production scale,the concentration under normal pressure is prior to selection,and mixed decoction according to theory of Traditional Chinese Medicine should be encouraged.Thereout,these above will provide good references for the safe use of Traditional Chinese Medicines containing aristolochic acids.

关 键 词：马兜铃酸 青木香 配伍 煎提 制备 

分 类 号：R283.1[医药卫生—中药学]