阳离子脂质体载药系统的制备及其体外成像治疗一体化应用研究  

Imaging and therapeutic platform of cancer cells with cationic liposome

作　　者：罗婷婷[1] 潘炜伦 涂嫣红 李博[1] 郑磊[1] LUO Tingting;PAN Weilun;TU Yanhong;LI Bo;ZHENG Lei(Department of Laboratory,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学南方医院检验科,广州510515

出　　处：《实用医学杂志》2022年第5期627-632,共6页The Journal of Practical Medicine

基　　金：国家杰出青年科学基金(编号:82025024)。

摘　　要：目的构建阳离子脂质体⁃阿霉素纳米复合物(CLPs⁃DOX)并将其应用于癌细胞成像及治疗一体化研究。方法本实验拟采用薄膜分散一锅法合成CLPs⁃DOX;利用电位分析仪、荧光分析仪、纳米颗粒追踪技术、荧光显微技术分析CLPs⁃DOX的电位、荧光强度、粒径分布和癌细胞的成像效果;细胞毒性试验验证CLPs⁃DOX对乳腺癌细胞的杀伤效能。结果薄膜分散一锅法合成CLPs⁃DOX效率较高,DOX装载于CLPs的荧光响应值在Triton X⁃100处理裂解后升高;DOX与CLPs⁃DOX的荧光峰值均在590 nm;当CLPs⁃DOX量为1×10^(8)个时,癌细胞死亡率达80%;CLPs⁃DOX能够高效进入乳腺癌MDA⁃MB⁃231细胞中并呈现出红色荧光。结论通过薄膜分散一锅法合成了粒径分布良好、稳定性强、装载率高的CLPs⁃DOX复合物,具有高效杀伤乳腺癌MDA⁃MB⁃231细胞和细胞成像的功能,为临床药物成像和治疗提供思路。Objective The aim of this study was to construct cationic liposome based CLPs-DOX platform for cancer therapy and imaging. Methods In this experiment,CLPs-DOX was synthesized by thin film dispersion method. Zeta potential,size distribution and fluorescence of CLPs-DOX were analyzed by potentiometric analyzer,fluorescence analyzer,nanoparticle tracking technology. The imaging effect,cytotoxicity of CLPs-DOX on breast cancer cells MDA-MB-231 were also investigated by fluorescence microscopy,confocal microscopy and CCK-8assay. Results DOX encapsulated efficiency of one-pot synthesis method was higher than two-step method. The fluorescence response value of DOX loaded on CLPs increased after Triton X-100 treatment. The fluorescence peaks of DOX and CLPs-DOX were both 590 nm. Cancer cells mortality rate increased to 20% in the addition of 1 × 10^(8)particles CLPs-DOX. CLPs-DOX showed a good imaging effect in breast cancer cells MDA-MB-231. Conclusion The synthesized CLPs-DOX exhibits desirable cytotoxicity and imaging capacity with uniform size distribution and high loading efficiency,paving the way to clinical practice.

关 键 词：乳腺癌 成像 治疗 阳离子脂质体 阿霉素 

分 类 号：R459.9[医药卫生—治疗学] R445.9[医药卫生—临床医学]