氯化胆碱/尿素低共熔溶剂提取桑树叶中黄酮研究  

Extraction of Flavonoids from Mulberry Leaves with Choline Chloride/Urea in Deep Eutectic Solvent

作　　者：宋亚宁 赵春晖 纪玉涵 辛炳炜 SONG Ya-ning;ZHAO Chun-hui;JI Yu-han;XIN Bing-wei(School of Chemistry and Chemical Engineering,Dezhou University,Dezhou Shandong 253023,China)

机构地区：[1]德州学院化学化工学院,山东德州253023

出　　处：《德州学院学报》2022年第2期28-30,39,共4页Journal of Dezhou University

基　　金：山东省教育教学研究课题(2020JXZ003);教育部产学合作协同育人项目(202002079042);2021年大学生创新创业训练计划(S202110448037,X202110448016,X202110448042)。

摘　　要：以低共熔溶剂作为天然产物提取剂,在不同温度、加热时间、配料比的条件下,对桑树叶中的天然组分进行提取,并利用紫外分光光度计在特定波长下测定吸光度,通过芦丁标准曲线进行计算得到其中黄酮的提取率来探索提取的最佳条件。通过单因素和正交实验,研究发现其最佳提取条件:在10 mL氯化胆碱/尿素(1:2)DES-40%H_(2)O中,投入0.25 g桑叶粉末,60℃温度下提取1 h,黄酮提取率为13.75 mg/g。使用绿色无污染的低共熔溶剂来提取桑树叶中的天然成分,既实现了绿色提取,还提高了提取率,并有望在化学、食品、医药等领域实现其更大价值。Choline chloride(ChCl)/urea deep eutectic solvent(DES)is us ed as the natural product extractant.The natural components in the mulberry leaves are extracted under the conditions of different temperature,heating time and ingredient ratio.The standard curve of rutin was calculated to obtain the extraction rate of flavonoids to explore the best extraction conditions.The study found the best extraction conditions:adding 0.25 g of mulberry leaf powder in 6 mL choline chloride/urea(1:2)DES-40%H_(2)O,extracting for 1 hour at 60 ℃,the extraction rate of flavonoids was 13.75 mg/g.This experimental work uses a green nonpolluting solvent to extract natural components from mulberry leaves,which not only achieves green extraction,but also improves the extraction rate,and is expected to achieve greater value in the fields of chemistry,food,and medicine.

关 键 词：低共熔溶剂 黄酮 最佳提取温度 最佳提取时间 最佳物料比 

分 类 号：O629[理学—有机化学]