CD71与E-cadherin在丙酸睾酮诱导的前列腺组织中的表达  

Expression of CD71 and E-cadherin in prostate tissue induced by testosterone propionate

作　　者：张强[1] 杨晓亮 杨晓波[1] 师宏斌[1] 赵瑞宁[1] 张超[1] ZHANG Qiang;YANG Xiaoliang;YANG Xiaobo;SHI Hongbin;ZHAO Ruining;ZHANG Chao(Department of Urology,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Lingwu Traditional Chinese Medicine Hospital,Yinchuan 751400,China)

机构地区：[1]宁夏医科大学总医院泌尿外科,宁夏银川750004 [2]宁夏灵武市中医医院,宁夏银川751400

出　　处：《宁夏医学杂志》2022年第4期319-321,F0003,共4页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ17166)。

摘　　要：目的探讨转铁蛋白受体71(CD71)与钙黏附蛋白E(E-cadherin)在丙酸睾酮诱导的前列腺组织中的表达。方法将12只SD大鼠随机分为对照组(n=6)和模型组(n=6),模型组大鼠给予皮下注射1 mL[2.5 mg/(kg·d)],连续4周;对照组给予相同剂量生理盐水皮下注射,末次注射后在无菌低温下剖腹取前列腺,免疫组织化学方法检测前列腺组织中CD71与E-cadherin蛋白的定位,蛋白印迹检测CD71与E-cadherin蛋白的表达量。结果免疫组化见CD71与E-cadherin蛋白均表达在前列腺上皮细胞中的细胞膜及细胞质中;蛋白印迹检测可见CD71与E-cadherin蛋白在模型组表达量均显著高于对照组(P<0.05)。结论在丙酸睾酮诱导的前列腺组织中CD71与E-cadherin高表达。Objective To study the expression of CD71 and E-cadherin in prostate tissue induced by testosterone propionate.Methods Twelve SD rats were randomly divided into the control group(n=6)and the model group(n=6).The model group was given subcutaneous injection of 1ml 2.5 mg/(kg·d)for four weeks,while the control group was given subcutaneous injection of the same dose of normal saline.After the last injection,the prostate was extracted by laparotomy at sterile low temperature.The localizations of CD71 and E-cadherin protein in prostate tissue were detected by immunohistochemistry,and the expressions of CD71 and E-cadherin protein were detected by western blot.Results Immunohistochemistry showed that CD71 and E-cadherin proteins were all expressed in prostate epithelial cells.Western blot analysis showed that the expressions of CD71 and E-cadherin protein in the model group were significantly higher than that in the control group(P<0.05).Conclusion CD71 and E-cadherin are highly expressed in prostate tissue induced by testosterone propionate.

关 键 词：丙酸睾酮 前列腺组织 转铁蛋白受体71 钙黏附蛋白E 

分 类 号：R694[医药卫生—泌尿科学]