36例HBsAg阴性DNA阳性献血者血液标本的血清学特征分析  

Analysis on serological characteristics of blood specimens of 36 HBsAg negative DNA positive blood donors

作　　者：陈慧[1] 孙国栋[1] 田丰 张丽[1] 张毓[1] 李丽[1] CHEN Hui;SUN Guo-dong;TIAN Feng;ZHANG Li;ZHANG Yu;LI Li(The Blood Center of Handan,Hebei 056001,China;不详)

机构地区：[1]邯郸市中心血站,河北056001 [2]河北工程大学

出　　处：《医学动物防制》2022年第1期21-23,27,共4页Journal of Medical Pest Control

基　　金：河北省医学科学研究课题计划(20211269)。

摘　　要：目的探讨分析HBsAg阴性HBV DNA阳性献血者标本的血清学特征,为输血安全策略的制定提供依据。方法收集邯郸市中心血站2018年1月—2019年2月的无偿献血者血液标本107199份,首先采用两种酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)试剂进行血清学标志物检测,将检测结果为HBsAg阴性的血液标本进行核酸(定性)检测,最终得到36份HBsAg阴性HBV DNA阳性标本,再对其进行化学发光(CLIA)"两对半"检测,得到不同感染阶段出现的不同乙肝血清学表型。结果联合筛查出36份HBsAg阴性,但HBV DNA阳性血清标本。化学发光法重测其"两对半"感染模式为中单纯HBcAb+的12份,占33%;HBeAb及HBcAb同时阳性10份,占28%;HBsAb及HBcAb同时阳性5份,占14%;全阴性6份,占17%;HBsAb、HBeAb及HBcAb同时阳性1份,占3%;单纯HBsAb阳性2份,占5%。结论HBV血清标志物与HBV DNA检测有密切的相关性,联合检测对早期诊断HBV感染以及了解病毒的复制情况等具有极其重要的临床意义。Objective To explore and analyze the serological characteristics of blood specimens of HBsAg negative HBV DNA positive blood donors,and to provide basis for formulation of blood transfusion safety strategy.Methods A total of 107199 blood samples of voluntary blood donors in the Blood Center of Handan from January 2018 to February 2019 were collected,two types of enzyme linked immunosorbent assay(ELISA)reagents were firstly used to test serological markers,test results of HBsAg negative blood samples were used for nucleic acid(qualitative)test,36 cases of HBV DNA positive specimens with negative HBsAg were finally obtained,and then chemiluminescence(CLIA)“hepatitis B serologic”test was implemented to obtain different hepatitis B serological phenotypes at different stages of infection.Results Thirty-six patients were found to have negative HBsAg and positive HBV DNA sera by joint screening.Chemiluminescence method was applied to retest 12 case receiving“hepatitis B serologic test”with the infection mode of single HBcAb+,accounting for 33%;10 cases were positive for both HBeAb and HBcAb,accounting for 28%;there were 5 cases with both positive HBsAb and HBcAb,accounting for 14%;6 cases were all negative,accounting for 17%;1 case was found to be positive in all of HBsAb,HBeAb and HBcAb,accounting for 3%;there were 2 cases with simple positive HBsAb,accounting for 5%.Conclusion The serum markers of hepatitis B virus(HBV)are closely related to the quantitative test of HBV DNA,and combined test is of great clinical significance in the early diagnosis of HBV infection,the understandings on virus replication and other circumstances.

关 键 词：乙型肝炎 乙肝血清标志物 乙肝病毒DNA 献血人群 输血安全 分析 

分 类 号：R373.21[医药卫生—病原生物学]