榨菜根肿病常发地根际土壤微生物群落特征研究  

On Rhizosphere Soil Mirobial Community Diversity of Clubroot-Diseased and Healthy Brassica Juncea Var.Tumida Tsen et Lee

作　　者：刘烈花 龚杰 皮静 董鹏 况觅 李姗蓉 袁国明 丁伟[1] LIU Liehua;GONG Jie;PI Jing;DONG Peng;KUANG Mi;LI Shanrong;YUAN Guoming;DING Wei(College of Plant Protection, Southwest University, Chongqing 400715, China;Chongqing General Station of Agricultural Technology Extension, Chongqing 401121, China;Chongqing Anbang Agricultural Development Co., Ltd., Chongqing 400030, China)

机构地区：[1]西南大学植物保护学院,重庆400715 [2]重庆市农业技术推广总站,重庆401121 [3]重庆安邦农业发展有限公司,重庆400030

出　　处：《西南师范大学学报（自然科学版）》2022年第4期63-72,共10页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：重庆市科委技术创新与应用示范项目(cstc2018jscx-mszdX0047).

摘　　要：系统研究和分析榨菜根肿病常发地发病植株与健康植株土壤微生物群落的结构特征,解析其与榨菜根肿病发生的关系,以期为调控根际微生态防控榨菜根肿病提供理论依据.采用细菌16S rDNA和真菌ITS高通量测序技术,对榨菜根肿病病株和健株根际土壤微生物群落结构和组成进行分析;利用随机矩阵方法建立病株和健株两组样本土壤微生物群落的分子生态网络拓扑图.结果显示:榨菜根肿病常发地病株和健株根际土壤微生物群落之间差异有统计学意义;Alpha多样性分析显示,病株根际土壤微生物群落(包括细菌和真菌)丰富度显著高于健株根际土壤,但健株根际土壤真菌群落多样性显著高于病株根际土壤;分子生态网络分析表明,健株根际土壤微生物群落内具有更多的连接点和边缘,连接度更高,显示出物种间共生互作关系更为复杂.此外,通过网络拓扑图的中介中心性筛选出健株根际土壤中具有生防作用的核心物种,细菌Mucilaginibacter(OTU12403)和Variovorax(OTU11035),真菌Penicillium(OTU378,OTU1296)和Cryptococcus(OTU2623),它们可能在抑制榨菜根肿病和维护根际土壤健康中发挥着重要作用.研究表明:榨菜根肿病病株和健株根际微生物群落的组成和结构差异有统计学意义,因此调控土壤微生物群落是植物土传病害绿色防控中具有战略意义的一项重要措施.To study and analyze systematically the characteristics of the soil microbial community structure of the clubroot-diseased and healthy stem mustard,the relationship between the structure characteristics of the rhizosphere microorganisms of mustard roots and the occurrence of mustard root swelling were analyzed so as to provide a theoretical basis for the green prevention and control of clubroot in mustard.Based on the 16S rDNA/ITS gene high-throughput sequencing technology,the structure and composition of clubroot-diseased and healthy mustard rhizosphere soil microbial communities were also analyzed;the random matrix method was used to establish the molecular ecological network topology diagram of soil microbial community of two groups of samples:the diseased mustard and healthy mustard.There were significant differences between the diseased and healthy mustard rhizosphere soil microbial community in the place where clubroot disease often occurs.The Alpha diversity analysis showed that the richness of the rhizosphere soil microbial community(including bacteria and fungi)of clubroot-diseased mustard was significantly higher than that of healthy mustard,but the healthy mustard rhizosphere soil fungal community diversity was significantly higher than that of diseased mustard rhizosphere soil.Molecular ecological network analysis showed that healthy soil microbial communities had more nodes and edges,closer connections,and more symbiotic interactions.In addition,through the centrality of the network topology,the core species with biocontrol effects in healthy rhizosphere soils were screened out,such as the bacteria Mucillaginibacter(OTU12403)and Variovorax(OTU11035),the fungi Penicillium(OTU378,OTU1296)and Cryptococcus(OTU2623),which may play an important role in suppressing clubroot disease and maintaining the health of rhizosphere soil.The study shows that there are significant differences in the composition and structure of rhizosphere microbial communities between clubroot-diseased and healthy mustard.Therefore,r

关 键 词：榨菜根肿病 高通量测序 微生物群落 根际土壤 

分 类 号：S436.344[农业科学—农业昆虫与害虫防治] S432.1[农业科学—植物保护]