百合叶烧病发病过程叶片细胞和差异表达基因分析  

Changes in Cell Structure and Gene Expression of Lily Leaves with Necrosis

作　　者：葛金涛[1] 王江英[1] 汤雪燕[1] 孙明伟[1] 腾年军[2] 朱朋波[1] 赵统利[1] 吴秋月 邵小斌[1] GE Jintao;WANG Jiangying;TANG Xueyan;SUN Mingwei;TENG Nianjun;ZHU Pengbo;ZHAO Tongli;WU Qiuyue;SHAO Xiaobin(Lianyungang Academy of Agricultural Sciences,Lianyungang,Jiangsu 222000,China;College of Horticulture,Nanjing Agricultural University,Nanjing,Jiangsu 210095,China;Bureau of Agricultural and Rural Affairs of Haizhou District,Lianyungang,Jiangsu 222006,China)

机构地区：[1]连云港市农业科学院,江苏连云港222000 [2]南京农业大学园艺学院,江苏南京210095 [3]连云港市海州区农业农村局,江苏连云港222006

出　　处：《福建农业学报》2022年第2期224-232,共9页Fujian Journal of Agricultural Sciences

基　　金：连云港市农业科学院青年基金项目(QNJJ2004);江苏省政策引导类计划项目(苏北科技专项)(SZ-LYG202039);江苏省农业科技自主创新资金[CX(20)3025]。

摘　　要：【目的】明确百合叶烧病发病过程中百合叶片细胞结构的变化,探究百合叶烧病发病的分子调控机理。【方法】通过扫描电镜和透射电镜观察东方百合Tarrango正常叶片、轻度叶烧叶片和重度叶烧叶片超微结构。并通过比较东方百合Tarrango正常叶片、叶烧叶片、正常叶片喷钙和叶烧叶片喷钙4种处理的转录组测序数据,对差异表达基因进行序列对比和分类分析。【结果】百合叶片近轴面表皮细胞大小随叶烧程度的加深而减小,而液泡失水是导致表皮细胞体积缩小的原因。转录组测序共获得349537条unigenes,平均长度为513.25 bp。124405条unigenes获得注释,占unigenes总数的35.59%。共发现7185个差异表达基因,包括5860个特异差异表达基因和1325个共同差异表达基因。KEGG富集分析显示,“代谢途径”“丙酮酸代谢”“次生代谢产物的生物合成”和“光合作用生物的碳固定作用”的基因在4组试验处理都有富集。百合叶烧病发病过程中显著下调基因有FOLK、PLD1_2、ATPeF1B和KCS、CALM、ENO和pel等;而在叶烧叶片喷钙后,表达量上调的基因有CALM、CPK、EIN2、AUX1、PLD1_2和SORD等。【结论】缺钙是导致百合叶烧病的重要因素,由缺钙引起的液泡失水和细胞皱缩等病害表征可能受脱落酸、乙烯和生长素等激素调控。【Objective】Changes in the cell structure and gene expression of leaf caused by the pathogenesis of lily upper leaf necrosis(ULN)were studied to understand the molecular mechanism of the disease on Oriental Lily Tarrango(Lilium tarrango).【Methods】Ultrastructure of the leaves from normal as well as mildly and severely ULN-infected lily plants was examined under a scanning electron microscope(SEM)and a projection electron microscope(TEM).Transcriptome sequences of the leaf specimens with or without calcium spraying were compared to identify the differentially expressed genes.【Result】The size of epidermal cells on the adaxial leaf surface shrank as the ULN worsened with vacuolar water loss.A total of 7185 differentially expressed genes were identified that included 5860 specific and 1325 common differentially expressed genes.The KEGG enrichment analysis showed that the genes were enriched in the metabolic pathways,pyruvate metabolism,biosynthesis of secondary metabolites,and carbon fixation of photosynthetic organisms in all 4 groups of specimens with or without calcium spraying.FOLK,PLD1_2,ATPeF1B,KCS,CALM,ENO,and pel were significantly downregulated during the progress of ULN on the leaves.After the calcium spraying,CALM,CPK,EIN2,AUX1,PLD1_2,and SORD were upregulated.【Conclusion】Calcium deficiency was deemed to be the key factor that led to ULN on the lily plants.The deficiency produced the symptoms,such as vacuolar moisture loss and cellular shrinkage,might be regulated by the hormone metabolisms related to abscisic acid,ethylene,and auxin.

关 键 词：百合 叶烧病 电镜 转录组 差异表达基因 

分 类 号：S682.29[农业科学—观赏园艺]