Evaluation of biogenic markers-based phenotyping for resistance to Aphanomyces root rot in field pea  

作　　者：Afef Marzougui Abirami Rajendran D.Scott Mattinson Yu Ma Rebecca J.McGee Manuel Garcia-Perez Stephen P.Ficklin Sindhuja Sankaran 

机构地区：[1]Department of Biological Systems Engineering,Washington State University,Pullman,WA,United States [2]Department of Horticulture,Washington State University,Pullman,WA,United States [3]United States Department of Agriculture-Agricultural Research Service,Grain Legume Genetics and Physiology Research Unit,Washington State University,Pullman,WA,United States

出　　处：《Information Processing in Agriculture》2022年第1期1-10,共10页农业信息处理（英文）

基　　金：This activity was funded in part by the U.S.Department of Agriculture-National Institute for Food and Agriculture(USDA-NIFA)Agriculture and Food Research Initiative(AFRI)Competitive Project WNP06825(ac-cession number 1011741);Hatch Project WNP00011(accession number 1014919);CAHNRS Emerging Research Issues project.

摘　　要：Biogenic volatile organic compounds(VOCs)emitted by plants can reveal information about plant adaptation,defense processes,and biological pathways.Thus,such VOC data may be utilized to capture phenotypic plant responses to the environment.In this study,the main objective was to evaluate the potential of biogenic compounds,including VOCs,to phenotype two pea cultivars,Ariel(susceptible)and Hampton(high levels of partial resistance)for resistance to Aphanomyces root rot disease.Plants were monitored non-destructively for VOC emission at three-time points(15,20,and 30 days after inoculation,DAI)using dynamic headspace sampling with gas chromatography-flame ionization detec-tion(GC-FID)system,as well as destructively at the end of the experiments,using solvent extraction and pyrolysis of both shoot and root tissues.A non-inoculated control(mock-inoculated with distilled water)was utilized to compare the plant responses within a cul-tivar.The common chemical peaks between control and inoculated samples of both culti-vars(RT_(cm))were analyzed after normalizing the relative peak intensity of inoculated samples with those of control samples,prior to a comparison between cultivars.In addi-tion,unique chemical peaks(RT_(uq))present in inoculated samples,but not in control sam-ples were also identified and their relative peak intensities were compared.Among the released green leaf volatiles(RT_(cm)),the normalized relative peak intensity of hexanal emis-sion,at 20 DAI,was higher in Ariel than that of Hampton.In addition,several putative chemical peaks(both RT_(cm) and RT_(uq)),previously known as indicators for disease response,exhibited some differences in their emission rates between pea cultivars in at least one of the time points.The destructive sampling revealed that shoot samples produced more putative unique biomarkers(RT_(uq))than the root samples.Based on the differences in puta-tive chemical peaks between cultivars,this initial study supports the concept of utilization of biogenic biomarker-based phenotyping in d

关 键 词：Plant breeding Pulse crop Volatile organic compounds Plant phenotyping Biotic stress 

分 类 号：S435[农业科学—农业昆虫与害虫防治]