人参皂苷Rd对非家族性高血脂SD大鼠血管保护的作用机制  

Protective effect of ginsenoside Rd on blood vessels of non-familial hyperlipidemia SD rats

作　　者：呼延丽[1] 张秀娟[1] 周进 高进贤[1] 朱菊花[1] 董彦富[1] Huyan Li;Zhang Xiu-juan;Zhou Jin;Gao Jin-xian;Zhu Ju-hua;Dong Yan-fu(Department of Pharmacy,Gansu Provincial Hospital,Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2022年第3期23-27,31,共6页Journal of Lanzhou University（Medical Sciences）

基　　金：甘肃省自然科学基金资助项目(21JRA626);甘肃省人民医院院内科研基金资助项目(18GSSY4-34)。

摘　　要：目的探讨人参皂苷Rd对非家族性高血脂SD大鼠血管保护作用及机制。方法采用全自动生化仪分析、病理学观察、实时荧光定量聚合酶链式反应、蛋白免疫印迹、酶联免疫吸附测定分别在生化、组织水平上检测。结果与高脂饲料组相比,人参皂苷Rd不同剂量组(30、35、40、45 mg/kg)大鼠血清中胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白含量下降、动脉粥样硬化指数改善、血管壁厚度降低、血管腔面积改善;人参皂苷Rd不同剂量组血管组织中一氧化氮合酶、卵磷脂胆固醇酰基转移酶、胆固醇7-羟化酶mRNA和蛋白表达均显著上调;血清中NO含量增加,而血浆内皮素、血管性血友病因子含量明显下降。结论人参皂苷Rd对非家族性高血脂SD大鼠血管具有保护作用,呈剂量依赖性。Objective To explore the protective effect of ginsenoside Rd on SD rats with non-familial hyperlipidemia.Methods Analyzed the biochemical,tissue levels with an automatic biochemical analyzer,pathological observation,quantitative PCR,Western blotting,and ELISA.Results Compared with the highfat diet group,the levels of cholesterol,triglycerides,low density lipoprotein,high density lipoprotein in the serum of SD rats in the DRU(30,35,40,and 45 mg/kg)groups decreased.Atherosclerosis index improved,blood vessel wall thickness decreased,and blood vessel lumen area improved.Ginsenoside Rd enhanced the levels of mRNA and protein of endothelial nitric oxide synthase,lecithin cholesterol acyltransferase,cholesterol7-hydroxylase in a dose-dependent relationship,and the concentration of serum NO increased,while the levels of endothelin and von Willebrand factor significantly decreased.Conclusion Ginsenoside Rd has a protective effect on the blood vessels of SD rats with non-familial hyperlipidemia in a dose-dependent manner.

关 键 词：人参皂苷RD 非家族性 内皮型一氧化氮合酶 卵磷脂胆固醇酰基转移酶 胆固醇7-羟化酶 

分 类 号：R446.1[医药卫生—诊断学]