MTA对脂多糖诱导人牙髓细胞分泌IL-6的影响  

Influence of MTA on the effect of lipopolysaccharide upon the secretion of IL-6 from human dental pulp cells

作　　者：侯雯[1] 刘婷婷[2] 阙国鹰 Hou Wen;Liu Ting-ting;Que Guo-ying(Department of Stomatology,Affiliated Beijing Ditan Hospital of Capital Medical University,Beijing 100015,China;Stomatological,Department of Stomatology,Affiliated Hospital of Binzhou Medical University,Binzhou 256603,Shandong,China;Stomatological Hospital of Southern Medical University,Guangdong Provincial Stomatology Hospital,Guangzhou 510260,China)

机构地区：[1]首都医科大学附属北京地坛医院口腔科,北京100015 [2]滨州医学院附属医院口腔科,山东滨州256603 [3]南方医科大学口腔医院广东省口腔医院,广东广州510260

出　　处：《兰州大学学报（医学版）》2022年第3期43-48,共6页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的探讨不同质量浓度的三氧化矿物凝聚体(MTA)对脂多糖(LPS)诱导人牙髓细胞(HDPC)分泌白细胞介素-6(IL-6)的影响,为MTA应用于牙髓细胞炎性损伤后再修复提供实验依据。方法采用改良组织块酶消化法原代培养HDPC,取第4代HDPC,经质量浓度为1μg/mL的LPS致炎24 h,分别与不同质量浓度的MTA浸提液(0.002、0.02、0.2、2、20 mg/mL)共同培养,ELISA法检测IL-6含量,并进行统计学分析。苏木精—伊红染色,电镜下观察细胞形态变化。结果5种质量浓度的MTA浸提液对LPS诱导HDPC分泌IL-6的水平均低于实验对照组(P<0.05)。MTA作用后,受损的HDPC细胞外形有恢复。结论MTA对LPS诱导HDPC分泌IL-6有抑制作用,在一定范围内随着MTA质量浓度的增加和作用时间的延长,抑制效果更好。Objective It is observed that the influence of mineral trioxide aggregate(MTA)in different doses on the effect of lipopolysaccharide(LPS)upon the secretion of interleukin-6(IL-6)from human dental pulp cells(HDPC),to provide experimental basis for the application of MTA in the repair of inflammatory injury of dental pulp cells.Methods The improved tissue explant enzymatic digestion was used for primary culture of HDPC.The 4 th generation HDPC was induced by LPS at the concentration of 1μg/mL for 24 hours,and cultured with different concentrations of MTA extracts(0.002、0.02、0.2、2.0、20 mg/mL)respectively.The levels of IL-6 were examined by ELISA,the results of which were statistically analyzed.The HE staining results were observed by the microscope.Results The levels of IL-6 secreted by HDPC induced by LPS in5 concentrations of MTA were lower than those in the control group(P<0.05).After the effect of MTA,the shape of damaged HDPC recovered.Conclusion MTA can inhibit IL-6 secretion of HDPC induced by LPS.In a certain range,the inhibition effect will be better with the increase of MTA concentration and the prolongation of action time.

关 键 词：人牙髓细胞 三氧化矿物凝聚体 脂多糖 白细胞介素-6 

分 类 号：R446.1[医药卫生—诊断学]