荷包牡丹的组培快繁技术研究  

Study on Tissue Rapid Propagation of Lamprocapnos spectabilis'Amore Rose'

作　　者：魏进莉 WEI Jinli(Beijing Florascape Co.,Ltd.,Beijing 100093,China)

机构地区：[1]北京市花木有限公司,北京100093

出　　处：《天津农业科学》2022年第4期10-14,共5页Tianjin Agricultural Sciences

摘　　要：为解决荷包牡丹(Lamprocapnos spectabilis'Amore Rose')的快繁问题,以荷包牡丹试管苗的叶片为外植体材料,进行了组培快繁技术的试验研究,建立了快繁技术体系。结果表明,以幼嫩新叶为外植体材料,更有利于愈伤组织及丛生芽的形成;初代培养基以MS+0.5 mg·L^(-1)6-BA+0.5 mg·L^(-1)KT+0.1 mg·L^(-1)NAA为最优,丛生芽的诱导率为85.7%;继代增殖培养基以MS+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA为最优,增殖系数达3.5;生根培养基以1/2 MS+0.5 mg·L^(-1)NAA+0.5 mg·L^(-1)IBA为最优,生根率达到98%;在草炭∶珍珠岩∶蛭石=7∶3∶2的优质移栽基质上,炼苗成活率达95%以上。最适宜的培养温度为20±1℃。用荷包牡丹的幼嫩试管苗叶片,通过愈伤组织诱导获得再生植株,成功建立了组培快繁技术体系。In order to solve the problem of rapid propagation of Lamprocapnos spectabilis'Amore Rose',the leaves was used as the explants to conduct the research of tissue culture techniques of propagation,and establish the sterile rapid propagation system in vitro.The results showed that it was easier to form callus and buds with young new leaves as explants.MS+0.5 mg·L^(-1)6-BA+0.5 mg·L^(-1)KT+0.1 mg·L^(-1)NAA was the suitable medium for explants induction of callus and cluster buds,the induction rate of cluster buds was 85.7%;MS+0.5 mg·L^(-1)6-BA+0.05 mg·L^(-1)NAA was the optimizing medium for multipropagation,the multiplication coefficient could reach up to 3.5;1/2 MS+0.5 mg·L^(-1)NAA+0.5 mg·L^(-1)IBA was the best medium for rooting,the rooting rate could reach to 98%.After 30 days transplanted into greenhouse,the survival rate of young plants were over 95%.The optimum culture temperature was 20±1℃.Regenerated plants were obtained from the leaves of young test tube plantlets of Lamprocapnos spectabilis'Amore Rose'through callus induction,and the technical system of tissue culture and rapid propagation was successfully established.

关 键 词：荷包牡丹 幼嫩新叶 丛生芽 组织培养 玻璃化 

分 类 号：Q943.1[生物学—植物学]