金属卟啉-Sn网络可控非共价功能化碳纳米管的制备及蛋白吸附应用  

Preparation of Controllable Non-covalent Functionalized Carbon Nanotubes with Metalloporphyrin-Sn Network and Application to Protein Adsorption

作　　者：王超峰 郑国栋 王悦 宋慧佳 陈小艺 高瑞霞[1] Wang Chaofeng;Zheng Guodong;Wang Yue;Song Huijia;Chen Xiaoyi;Gao Ruixia(School of Chemistry,Xi'an Jiaotong University,Xi'an 710049,China;School of Pharmacy,Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学化学学院,西安710049 [2]西安交通大学药学院,西安710061

出　　处：《化学学报》2022年第2期126-132,共7页Acta Chimica Sinica

基　　金：陕西省自然科学基金(No.2020JM-066)资助.

摘　　要：本工作通过简便的一锅溶剂热法,发展一种卟啉一步金属化且与Sn桥连形成的配合物网络非共价功能化的多壁碳纳米管复合材料(MMPT),以增强对蛋白质的吸附.在溶剂热条件下,Sn进入中位-四(4-羧基苯基)卟吩(TCPP)大环使卟啉金属化形成SnTCPP,同时Sn作为交联剂桥连多壁碳纳米管(MWCNTs)表面的SnTCPP分子,通过配位自组装形成均匀连续的SnTCPP-Sn网络壳层,而无需对TCPP和/或MWCNTs进行任何的修饰.在这种合成策略下,通过调整MWCNTs/TCPP的比例,实现了卟啉层厚和位点密度的可控.并通过扫描电子显微镜(SEM)、透射电子显微镜(TEM)及其能量色散X射线能谱(EDS)、紫外-可见分光光度计(UV-Vis spectrophotometer)、傅里叶变换红外分光光度计(FT-IR spectrophotometer)、zeta电位等对所得MMPT的结构和性能进行了全面的表征.在最佳的碳纳米管比例和吸附条件下,MMPT对牛血清白蛋白(BSA)表现出较好的吸附性能,此外,MMPT对其他蛋白质如牛血红蛋白(BHb)和溶菌酶(Lyz)也表现出良好的吸附亲和力,使得其在蛋白吸附、药物递送、传感等方面具有广泛的应用前景.In order to enhance the adsorption of protein,a one-step metallization of porphyrin and a novel metalloporphy-rin-Sn network non-covalently functionalized multi-walled carbon nanotube composite(MMPT),were developed only by a simple one-pot solvothermal method.Under solvothermal conditions,Sn entered the meso-tetrakis(4-carboxyphenyl)porphine(TCPP)macrocycle to metalize the porphyrins and formed SnTCPP.Meanwhile,Sn,as a crosslinker,bridged the SnTCPP molecules around the surface of multi-walled carbon nanotubes(MWCNTs)to form a uniform and continuous SnTCPP-Sn network layer through self-assembly without any pre-modification for TCPP and MWCNTs.Then,throughπ-πstacking,multilayers of SnTCPP-Sn network can be formed via layer-by-layer self-assembly,as well as MMPT with controllable layer thickness.With this synthetic strategy,the sites of porphyrin on the MWCNTs surfaces were found continuous and evenly distributed,which enabled the controllability of layer thickness as well as the site density by adjusting the MWCNTs/TCPP ratio.The morphology,structure and properties of MMPT were characterized comprehensively by scanning electron micro-scope(SEM),transmission electron microscope(TEM)and its energy-dispersive X-ray spectroscopy(EDS),ultraviolet-visible(UV-Vis)spectroscopy,Fourier transform infrared spectrophotometer(FT-IR),and zeta potential.In addition,the ef-fects of various factors on its adsorption properties and the interaction between MMPT and bovine serum albumin(BSA)were investigated.When the mass ratio of MWCNTs was 1.0 and the concentration of BSA was 1.5 mg·g^(−1),the effective adsorption sites per unit mass of MMPT reached the maximum,resulting in the maximum adsorption capacity of MMPT to BSA(651.4 mg·g^(−1)),and the adsorption equilibrium can be reached in only 5 min.Compared with other adsorbents,MMPT showed high adsorption capacity for BSA under the optimal carbon nanotube ratio and adsorption conditions.In addition,MMPT also exhibited a good adsorption affinity for other proteins like bovine

关 键 词：卟啉 碳纳米管 金属化 非共价 一锅法 蛋白吸附 

分 类 号：TB383.1[一般工业技术—材料科学与工程] TQ127.11[化学工程—无机化工] O647.3[理学—物理化学]