杨山牡丹生防内生细菌的分离及鉴定  

Isolation and Identification of Endophytic Bacteria from Paeonia ostii

作　　者：刘雪停 夏彦飞[1,2] 李身 雷荣月 徐建强 林晓民[1] LIU Xue-ting;XIA Yan-fei;LI Shen;LEI Rong-yue;XU Jian-qiang;LIN Xiao-min(Henan University of Science and Technology,Luoyang 471023,China;Affilliated Hospital of Guilin Medical College,Guilin 541001,China)

机构地区：[1]河南科技大学,河南洛阳471003 [2]桂林医学院附属医院,广西桂林541001

出　　处：《江西农业学报》2022年第2期62-68,共7页Acta Agriculturae Jiangxi

基　　金：国家自然科学基金项目(31201558);河南省教育厅高等学校重点科研项目(19A210010)。

摘　　要：为了丰富生防微生物菌种资源库,从杨山牡丹的根、茎、叶中共分离出211株内生细菌,经初筛和复筛,有6株菌株(YMG34、YMJ74、YMJ78、YMJ141、YMJ143、YMY13)对牡丹黑斑病菌(Alternaria suffruticosae)、黄斑病菌(Phyllosticta commonsii)、腔孢叶斑病菌(Hainesia lythri)、灰霉病菌(Botrytis paeoniae)和北方根结线虫(Meloidogyne hapla)表现出稳定的拮抗活性。拮抗实验结果表明,6株内生菌处理能使牡丹病原真菌菌丝生长扭曲,出现泡囊结构以及局部膨大。经生理生化实验并结合16S rDNA及gyrB基因序列分析,YMG34菌株被鉴定为巨大芽孢杆菌(Bacillus megaterium);其余5个菌株被鉴定为耐盐芽孢杆菌(B.halodurans),结果表明6株内生细菌菌株均具有防治牡丹病害的潜力。In order to enrich the resource library of biocontrol microbial species,211 endophytic bacterial strains were isolated from the root,stem and leaf of Paeonia ostii.After initial screening and rescreening,six strains(YMG34,YMJ74,YMJ78,YMJ141,YMJ143 and YMY13)showed the stable antagonistic activity to Alternaria suffruticosae,Phyllosticta commonsii,Hainesia lythri,Botrytis paeoniae and Meloidogyne hapla.The antagonism test showed that the mycelia of pathogenic fungi treated with six endophytic bacteria showed abnormal growth,twist,bubble deformation structures and differential expansion.Based on physiological and biochemical characters,16S rRNA and gyrB gene sequence analysis,YMG34 was identified as Bacillus megaterium,and the other strains were identified as B.halodurans.The results demonstrated the biocontrol potential of 6 endophytic bacteria against peony diseases.

关 键 词：杨山牡丹 内生细菌 生物防治 分离鉴定 

分 类 号：S476.19[农业科学—农业昆虫与害虫防治]