电导率法评价紫云英种子活力并预测田间出苗表现  

Evaluation of Seed Vigor and Prediction of Field Seedling Emergence of Chinese Milk Vetch(Astragalus sinicus L.)by Conductivity Method

作　　者：陶奇波 孙继鹏 聂宇婷 张亚锜 孙小同 牛天秀 唐伟 杨超 孙娟[1] TAO Qi-bo;SUN Ji-peng;NIE Yu-ting;ZHANG Ya-qi;SUN Xiao-tong;NIU Tian-xiu;TANG Wei;YANG Chao;SUN Juan(College of Grassland Science,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]青岛农业大学草业学院,山东青岛266109

出　　处：《中国草地学报》2022年第4期95-103,共9页Chinese Journal of Grassland

基　　金：国家牧草产业技术体系(CARS-34);山东省一流学科—草学(1619002);青岛农业大学高层次人才启动基金(6631121038)共同资助。

摘　　要：以不同种源紫云英种子为材料,分析浸种3、6、12、24、36、48和60h的种子浸出液的绝对和相对电导率,并测定各样品的标准发芽率和田间出苗表现,以期探求适宜于紫云英种子的活力评价技术。12个紫云英样品的标准发芽率在87%~97%之间,且回归分析表明其和田间出苗率及田间幼苗干重无显著相关性,无法准确反映各种子样品活力水平。随浸种时间增加,种子浸出液的绝对和相对电导率呈上升趋势,且和田间出苗表现的相关性逐渐增强。回归分析结果表明在浸种24h,种子浸出液的绝对和相对电导率与田间出苗率存在极显著负相关关系,回归分析决定系数分别为0.897和0.876;随浸种时间继续延长,相关性不再显著增加。因此,浸种24h的种子浸出液的绝对或相对电导率均可以作为评价紫云英种子活力且预测田间出苗表现的适宜方法,且能够在1d完成测定,兼具精确性与时效性。This study measured seed leachate’s absolute and relative electrical conductivities after soaking for 3,6,12,24,36,48 and 60 hours for 12 samples of Chinese milk vetch(Astragalus sinicus L.).The germination percentage and field seedling emergence were also determined to explore the suitable method for seed vigor evaluation.The results showed that the standard germination percentage of Chinese milk vetch ranged from 87%to 97%.Moreover,regression analysis revealed no significant correlation between standard germination percentage,field seedling emergence percentage,and seedling dry weight in field,indicating that standard germination percentage was not an appropriate indicator for seed vigor determination.With the increase of soaking time,both absolute and relative conductivities of seed leachate increased,and their correlation with field seedling emergence also elevated.The regression analysis revealed that both absolute and relative conductivities negatively correlated with field seedling emergence percentage after soaking for 24 hours,in which the determination coefficients were 0.897 and 0.876,respectively.With the increase of soaking time(longer than 24 hours),the correlation between conductivities and field seedling emergence percentage was improved insignificantly.Therefore,absolute or relative conductivity of seed leachate after soaking for 24 hours could be used as an optimum vigor evaluation method to accurately and efficiently predict field seedling emergence of Chinese milk vetch.

关 键 词：标准发芽率 电导率 田间出苗 种子活力 紫云英 

分 类 号：S541.3[农业科学—作物学]