红景天苷对97H细胞自噬的影响  

Salidroside induces the autophagy of 97H cells

作　　者：蒋兵 杨韬[1] 封龙飞 王涛[2] 苏海翔[1,2] Jiang Bing;Yang Tao;Feng Longfei;Wang Tao;Su Haixiang(Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China;Gansu Provincial Academic Institute for Medical Research,Gansu Provincial Cancer Hospital,Lanzhou 730050,China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省医学科学研究院/甘肃省肿瘤医院,甘肃兰州730050

出　　处：《甘肃医药》2022年第3期193-197,共5页Gansu Medical Journal

基　　金：甘肃省肿瘤分子病理诊断临床医学研究中心项目(项目编号:18JR2FA010);甘肃省2021年度重点人才项目(文件号:甘组通(2021)17号);甘肃省中医药管理局课题(项目编号:GZKP-2021-21)。

摘　　要：目的:探讨红景天苷对人高转移性肝癌细胞株97H自噬的影响。方法:97H细胞经红景天苷处理后,采用透射电镜、MDC染色和吖啶橙AO染色观察自噬现象;采用qRT-PCR方法检测自噬相关基因的mRNA水平;用Western blot方法检测自噬相关蛋白的表达。结果:红景天苷可诱导97H细胞发生自噬,且上调Beclin-1基因mRNA水平,下调p62基因mRNA水平(P<0.05),上调Beclin-1和LC3-Ⅱ/Ⅰ蛋白的表达,下调p62蛋白的表达(P<0.05)。结论:红景天苷可以诱导97H细胞的自噬作用。Objective:To study the inducing effect of salidroside on the autophagy of human highly metastatic liver cancer cells(97H cells). Methods:97H cells were treated with salidroside,then transmission electron microscopy,MDC staining and AO staining were used to observe the phenomenon of autophagy,the qRT-PCR was used to detect the mRNA levels of autophagy-related genes,western-blot was used to detect the expression of autophagy-related proteins. Results:Salidroside induces the autophagy in 97H cells,up-regulates the mRNA level of Beclin-1 gene,down-regulates the mRNA level of p62 gene(P<0.05),up-regulates the expression of Beclin-1 and LC3-Ⅱ/Ⅰ protein,and down-regulates the expression of p62 protein(P<0.05). Conclusion:Salidroside can induce autophagy in 97H cells.

关 键 词：红景天苷 肝癌 肝癌细胞株 97H 自噬 

分 类 号：R285[医药卫生—中药学]