MALDI-TOF MS用于耐碳青霉烯类阴沟肠杆菌的同源性分析  

Homology analysis of carbapenem resistant Enterobacter cloacae by MALDI-TOF MS

作　　者：张河林 张敏[2] 赵秀林 赵平森 ZHANG Helin;ZHANG Min;ZHAO Xiulin;ZHAO Pingsen(Department of Clinical Laboratory,Yuebei People′s Hospital,Shaoguan,Guangdong 512026,China;School of Clinical Medicine,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510030,China;Department of Gastrointestinal Surgery,Yuebei People′s Hospital,Shaoguan,Guangdong 512026,China)

机构地区：[1]粤北人民医院检验科,广东韶关512026 [2]广东药科大学临床医学院,广东广州510030 [3]粤北人民医院胃肠外科,广东韶关512026

出　　处：《检验医学与临床》2022年第8期1014-1017,共4页Laboratory Medicine and Clinic

基　　金：国家重点研发计划项目(2016YFD0500405,2017YFD0501705);广东省自然科学基金项目(2021A1515012429);广东省医学科研基金立项课题(B2019205);广东省韶关市医药卫生科研计划项目(Y19047)。

摘　　要：目的以脉冲场凝胶电泳(PFGE)为金标准,评价临床实验室应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)对耐碳青霉烯类阴沟肠杆菌(CRECL)进行同源性分析的可行性。方法收集2018年8月至2019年6月从粤北人民医院住院患者中分离的非重复CRECL菌株10株,编号YG1~YG10。利用Biotyper 3.1软件对CRECL蛋白图谱进行主成分分析(PCA)和核心图谱(MSP)聚类分析,同时进行PFGE同源性分析,并将2种质谱分型方法所得的结果与金标准对比分析。编号为YG10的菌株无法进行PFGE分型被排除。结果PFGE同源性分析结果与MALDI-TOF MS同源性分析结果有较大差异,PFGE分型结果显示9株CRECL为不同型;MSP聚类分析将9株CRECL分为3型(A型4株,B型4株,C型1株),其中A型的YG3和YG7,以及B型的YG2和YG5水平线距离均为0;PCA聚类分析同样将9株CRECL分为3型(A型2株,B型4株,C型3株)。2种质谱聚类分析都将YG3和YG6分到同一型中,而YG2、YG4及YG8分到另外的同一型中。结论MALDI-TOF MS有一定的同源性分型能力,但与PFGE同源性分型结果相比较,MALDI-TOF MS的2种同源性分型结果都还有待进一步研究。Objective To evaluate the feasibility of matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)for homology analysis of carbapenem resistant Enterobacter cloacae(CRECL)in clinical laboratory using pulsed field gel electrophoresis(PFGE)as the gold standard.Methods Ten non-repetitive CRECL strains isolated from inpatients in Yuebei People′s Hospital from August 2018 to June 2019 were collected and numbered YG1-YG10.The principal component analysis(PCA)and main spectra profile(MSP)cluster analysis of CRECL protein maps were performed by Biotyper 3.1 software,and the PFGE homology analysis was also performed.The results of the two mass spectrometry typing methods were compared with the gold standard.The strain numbered YG10 could not be PFGE typed and was excluded.Results The results of PFGE homology analysis and MALDI-TOF MS homology analysis showed that there were significant differences between the two methods.The results of PFGE typing showed that 9 strains of CRECL were different types.Nine strains of CRECL were divided into 3 types(4 strains of type A,4 strains of type B,and 1 strain of type C)by MSP cluster analysis.Among them,the horizontal line distances of YG3 and YG7 of type A,and YG2 and YG5 of type B were all 0.Nine strains of CRECL were also divided into 3 types(2 strains of type A,4 strains of type B,and 3 strain of type C)by PCA cluster analysis.YG3 and YG6 were classified into the same type by both mass spectrometry cluster analysis,while YG2,YG4 and YG8 were classified into another same type.Conclusion MALDI-TOF MS has certain ability of homology typing,but compared with PFGE homology typing results,the two homology typing results of MALDI-TOF MS need further studies.

关 键 词：基质辅助激光解吸电离飞行时间质谱 脉冲场凝胶电泳 耐碳青霉烯类阴沟肠杆菌 同源性分析 

分 类 号：R446.5[医药卫生—诊断学]