井穴放血减轻全身炎症反应防治大鼠心脏骤停后脑损伤  

Bloodletting at Jing-Well Points to Reduce General Inflammatory Reactions in Preventive Treatment of Brain Injury After Sudden Cardiac Arrest in Rats

作　　者：钟晓芃[1] 郭义[2] ZHONG Xiaopeng;GUO Yi(Tianjin People’s Hospital,Tianjin 300121,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津市人民医院,天津300121 [2]天津中医药大学,天津301617

出　　处：《上海针灸杂志》2022年第4期406-412,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金重点项目(82030125);天津市卫健委课题(2019121)。

摘　　要：目的探讨十二井穴放血疗法对大鼠心脏骤停后脑损伤的保护作用及其机制。方法18只SD大鼠随机分配到假手术组、模型组和井穴组3组,每组6只。假手术组不诱导心脏骤停,模型组采用气管夹闭法诱导心脏骤停后进行心肺复苏,井穴组在模型组基础上加用手十二井穴刺络放血。观察不同时间点神经功能缺陷评分、血清IL-6、TNF-α水平,并对72 h后处死大鼠取大脑海马组织进行尼氏染色及TUNEL法检测细胞凋亡。结果复苏后模型组和井穴组神经功能缺陷评分均较假手术组显著下降(P<0.05),其中6 h最为明显,至72 h井穴组和假手术组比较,差异无统计学意义(P>0.05)。复苏后72 h尼氏染色显示假手术组大鼠海马神经元细胞细胞核大,核仁明显;与模型组比较,井穴组大鼠脑组织神经元水肿较轻,细胞核形态较好。复苏后72 h时后,与假手术组比较,模型组和井穴组凋亡指数显著增高(P<0.05);与模型组比较,井穴组凋亡指数显著降低(P<0.05)。复苏后6 h后与假手术比较,模型组和井穴组血清IL-6、TNF-α水平显著升高(P<0.05)。与模型组比较,井穴组血清IL-6于复苏后24 h、72 h显著降低(P<0.05),血清TNF-α于复苏后24 h、48 h及72 h显著降低(P<0.05)。结论减轻心脏骤停后机体炎症反应可能是十二井穴刺络放血防治复苏后脑损伤的机制之一。Objective To discuss the protective effect of bloodletting therapy at the twelve Jing-Well points for brain injury after sudden cardiac arrest(SCA)in rats and its mechanism of action.Method Eighteen Sprague-Dawley(SD)rats were randomly assigned to a sham operation group,a model group,and a Jing-Well point group,with 6 rats in each group.The sham operation group did not undergo the induction of SCA.In the model group,SCA was induced by clipping the trachea,and then cardiopulmonary resuscitation was performed.The Jing-Well point group received bloodletting at the twelve Jing-Well points based on the model group.The neurological deficit score and serum interleukin(IL)-6 and tumor necrosis factor(TNF)-αlevels were observed at each time point.The rats were sacrificed 72 h later to collect the hippocampal tissues for Nissl staining and TUNEL to detect cell apoptosis.Result After resuscitation,the neurological deficit score decreased significantly in the model and Jing-Well point groups compared with the sham operation group(P<0.05),especially at 6 h,and until 72 h,there was no significant difference between the Jing-Well point and sham operation groups(P>0.05).At 72 h after resuscitation,Nissl staining showed large nuclei and notable nucleoli in the hippocampal neurons of rats in the sham operation group;compared with the model group,the Jing-Well point group had less severe edema in the brain neurons and better nuclear morphology.At 72 h after resuscitation,the apoptosis index increased significantly in the model and Jing-Well point groups compared with the sham operation group(P<0.05);compared with the model group,the apoptosis index decreased markedly in the Jing-Well point group(P<0.05).Compared with the sham operation group at 6 h after resuscitation,the levels of serum IL-6 and TNF-αincreased significantly in the model and Jing-Well point groups(P<0.05).Compared with the model group,the serum IL-6 level decreased notably at 24 h and 72 h after resuscitation(P<0.05),and the serum TNF-αlevel dropped significantl

关 键 词：放血疗法 穴 井(五输) 心跳骤停 脑损伤 细胞因子 大鼠 

分 类 号：R2-03[医药卫生—中医学]