基于HPLC法测定王不留行不同炮制品中主成分含量  

Determination of Main Components in Different Vaccaria Segetalis Products by HPLC

作　　者：黄检平 周鸣[2] 陈秀娟 李晶 高慧 叶喜德[2] HUANG Jianping;ZHOU Ming;CHEN Xiujuan;LI Jing;GAO Hui;YE Xide(Shanggao County Hospital of Traditional Chinese Medicine,Shanggao Jiangxi 336400,China;Jiangxi University of Chinese Medicine,Nanchang Jiangxi 330004,China;Changle District Maternal and Child Health Care Hospital,Fuzhou Fujian 350200,China)

机构地区：[1]上高县中医院,江西上高336400 [2]江西中医药大学,江西南昌330004 [3]福州市长乐区妇幼保健院,福建福州350200

出　　处：《药品评价》2022年第3期132-136,共5页Drug Evaluation

基　　金：江西省中医药中青年骨干人才(第一批)培养计划(赣中医药科教字[2020]2号)。

摘　　要：目的:测定王不留行生品、清炒品、润炒品、酒炒品及油制品中主要有效成分刺桐碱和王不留行黄酮苷的含量,为优选王不留行优质饮片提供科学依据。方法:色谱柱为Tnature C18(250 mm×4.6 mm,5 μm);流动相为乙腈-0.1%甲酸溶液,梯度洗脱;检测波长:280 nm;柱温:30 ℃;流速:1.0 mL/min,进样量:5 μL。结果:5种王不留行炮制品中,刺桐碱含量高低顺序为:生品>清炒品>酒炒品>润炒品>油制品;王不留行黄酮苷含量高低顺序为:生品>清炒品>酒炒品>润炒品>油制品。结论:王不留行不同炮制品中刺桐碱和王不留行黄酮苷含量存在差异。其中炮制对刺桐碱含量影响相对较小,而对王不留行黄酮苷的影响较大。Objective:A HPLC method was used to determine the contents of hypaphorine and vaccarin in raw,stir-fried,liquid,wine and oil products of vaccaria segetalis,and to compare the differences between them so as to provide a scientific basis for selecting the best quality pieces of vaccaria segetalis.Methods:Analysis was carried out on a Tnature C18 (250 mm×4.6 mm,5 μm) column with acetonitrile-0.1% formic acid water as mobile phase in gradient elution.The flow rate of the mobile phase,detection wavelength,column temperature and injection volume were set at 1.0 mL/min,280 nm,30 ℃ and 5 μL,respectively.Results:The contents of hypaphorine in different processing methods were as follows:raw products > clear fried products > wine fried products > moistening fried products > oil products.The contents of vaccarin in different processing methods were as follows:raw products > clear fried products > wine fried products > moistening fried products > oil products.Conclusion:Based on the HPLC method,there are differences in the contents of hypaphorine and vaccarin in different vaccaria segetalis processed products.According to the results,the processing have little effect on hypaphorine but great effect on vaccarin.

关 键 词：王不留行属 主成分分析 含量差异 炮制 HPLC 

分 类 号：O657.72[理学—分析化学] R283.1[理学—化学]