口腔液在新型冠状病毒核酸和抗体检测中的应用  

Application of oral fluid in SARS-CoV-2 nucleic acid and antibody detection

作　　者：黄琪 刘方遥[3] 毛乃颖[4,5] 孙静怡 董梅 谢会[2] 刘锋 张恒[3] 于霞丽 董建平[6] 许文波[4,5] 黄芳[1,2] Huang Qi;Liu Fangyao;Mao Naiying;Sun Jingyi;Dong Mei;Xie Hui;Liu Feng;Zhang Heng;Yu Xiali;Dong Jianping;Xu Wenbo;Huang Fang(School of Public Health,Capital Medical University,Beijing 100069,China;Institute of Preventive Immunization,Beijing Center for Disease Control and Prevention/Beijing Academy for Preventive Medicine/Beijing Institute of Tuberculosis Control Research and Prevention,Beijing 100013,China;Department of Microbiology Laboratory,Beijing Haidian Center for Disease Control and Prevention,Beijing 100094,China;Institute for Viral Disease Control and Prevention,Chinese Centers for Disease control and Prevention,Beijing 102206,China;NHC Key Laboratory for Medical Virology and Viral Diseases,Beijing 102206,China;Department Infectious Disease,Beijing Haidian Hospital,Beijing 100080,China)

机构地区：[1]首都医科大学公共卫生学院,北京100069 [2]北京市疾病预防控制中心免疫预防所北京市预防医学科学院北京市结核病控制研究与防治所,北京100013 [3]北京市海淀区疾病预防控制中心微生物检验科,北京100094 [4]中国疾病预防控制中心病毒病预防控制所,北京102206 [5]卫生部医学病毒和病毒病重点实验室,北京102206 [6]北京市海淀医院感染性疾病科,北京100080

出　　处：《中华预防医学杂志》2022年第3期355-359,共5页Chinese Journal of Preventive Medicine

基　　金：首都卫生发展科研专项(2021-1G-3011);首都卫生发展科研专项(2021-1G-3015)。

摘　　要：为探讨新型生物标本口腔液在新型冠状病毒(SARS-CoV-2)核酸和抗体检测中的应用价值,采集2021年10—11月北京两起新型冠状病毒肺炎(简称“新冠肺炎”)聚集性疫情的7例确诊病例的口腔液及配对的呼吸道和血液样本。采用荧光定量PCR和血清抗体检测试剂盒分别检测SARS-CoV-2核酸和IgG抗体,同时采用新建的磁微粒化学发光法检测口腔液标本SARS-CoV-2 IgG抗体。结果显示,3例新冠肺炎确诊病例鼻咽拭子、咽拭子和口腔液标本SARS-CoV-2核酸检测结果均为阳性,其中2例病例口腔液双基因循环阈值(Ct值)与咽拭子相近,1例高于咽拭子。获得1例确诊病例口腔液标本全基因组序列,属于VOC/Delta变异株。口腔液和配对血清标本SARS-CoV-2 IgG抗体均为阳性,口腔液的S/CO值较血清低。其中,采集到口腔液系列标本的病例,口腔液抗体水平在发病后11~32 d呈上升趋势。This study aimed to explore the application value of new biological specimen oral fluid in SARS-CoV-2 nucleic acid and antibody detection.Oral fluid and paired respiratory and blood specimens from 7 confirmed cases of two COVID-19 cluster epidemic were collected in Beijing from October to November 2021.SARS-CoV-2 virus and IgG antibody were detected by real time PCR kits and serum antibody detection reagents,and SARS-CoV-2 IgG antibody in oral fluids was detected by a new established method of magnetic particle chemiluminescence.The results showed that the nucleic acid amplification test of SARS-CoV-2 on nasopharyngeal swabs,throat swabs and oral fluid specimens from 3 confirmed cases of COVID-19 was positive,among which the Ct value for ORF1a/b and N gene of oral fluid samples in 2 cases was close to that of throat swab,and the Ct value of oral fluid sample for 1 case was higher than that of throat swab.The complete genome sequence of one oral fluid specimen was obtained,which belonged to the VOC/Delta variant strain.The SARS-CoV-2 IgG antibodies of the paired oral fluid and serum were all positive,and the S/CO values of oral fluid were all lower than those of serum.The series of oral fluid results showed that SARS-CoV-2 IgG antibody level increased from 11 to 32 days after the onset of the disease.

关 键 词：新型冠状病毒 核酸检测 抗体检测 口腔液 

分 类 号：R446.6[医药卫生—诊断学]