Avoiding artefacts in MicroCT imaging of collagen scaffolds:Effect of phosphotungstic acid(PTA)-staining and crosslink density  

作　　者：Kyung-Ah Kwon Daniel V.Bax Jennifer H.Shepherd Ruth E.Cameron Serena M.Best 

机构地区：[1]Cambridge Centre for Medical Materials,Department of Materials Science and Metallurgy,University of Cambridge,Cambridge,United Kingdom [2]School of Engineering,University of Leicester,Leicester,United Kingdom

出　　处：《Bioactive Materials》2022年第2期210-219,共10页生物活性材料（英文）

基　　金：supported by Engineering and Physical Sciences Research Council(EP/N019938/1).

摘　　要：X-ray micro-computed tomography(μ-CT)can be used to provide both qualitative and quantitative information on the structure of three-dimensional(3D)bioactive scaffolds.When performed in a dry state,μ-CT accurately reflects the structure of collagen-based scaffolds,but imaging in a wet state offers challenges with radiolucency.Here we have used phosphotungstic acid(PTA)as a contrast agent to visualise fully hydrated collagen scaffolds in a physiologically relevant environment.A systematic investigation was performed to understand the effects of PTA on the results of μ-CT imaging by varying sample processing variables such as crosslinking density,hydration medium and staining duration.Immersing samples in 0.3% PTA solution overnight completely stained the samples and the treatment provided a successful route forμ-CT analysis of crosslinked samples.However,significant structural artefacts were observed for samples which were either non-crosslinked or had low levels of crosslinking,which had a heterogeneous interior architecture with collapsed pores at the scaffold periphery.This work highlights the importance of optimising the choice of processing and staining conditions to ensure accurate visualisation for hydrated 3D collagen scaffolds in an aqueous medium.

关 键 词：MICROCT CROSSLINKING qualitative 

分 类 号：R318[医药卫生—生物医学工程]