Bioinspired supramolecular nanofiber hydrogel through self-assembly of biphenyl-tripeptide for tissue engineering  

作　　者：Yong Sun Xing Li Mingda Zhao Yafang Chen Yang Xu Kefeng Wang Shaoquan Bian Qing Jiang Yujiang Fan Xingdong Zhang 

机构地区：[1]National Engineering Research Center for Biomaterials,Sichuan University,29 Wangjiang Road,Chengdu,Sichuan,610064,PR China [2]Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,Shenzhen,518055,PR China [3]College of Materials Science and Engineering,Sichuan University,29 Wangjiang Road,Chengdu,Sichuan,610064,PR China

出　　处：《Bioactive Materials》2022年第2期396-408,共13页生物活性材料（英文）

基　　金：sponsored by National Key R&D Program of China(Grant No.2018YFC1105900);National Natural Science Foundation of China(32071352);Sichuan Province Key R&D Program(2019YFS0007);Sichuan university Innovation Spark Project(2018SCUH0089).

摘　　要：Supramolecular nanofiber peptide assemblies had been used to construct functional hydrogel biomaterials and achieved great progress.Here,a new class of biphenyl-tripeptides with different C-terminal amino acids sequences transposition were developed,which could self-assemble to form robust supramolecular nanofiber hydrogels from 0.7 to 13.8 kPa at ultra-low weight percent(about 0.27 wt%).Using molecular dynamics simulations to interrogate the physicochemical properties of designed biphenyl-tripeptide sequences in atomic detail,reasonable hydrogen bond interactions and“FF”brick(phenylalanine-phenylalanine)promoted the formation of supramolecular fibrous hydrogels.The biomechanical properties and intermolecular interactions were also analyzed by rheology and spectroscopy analysis to optimize amino acid sequence.Enhanced L929 cells adhesion and proliferation demonstrated good biocompatibility of the hydrogels.The storage modulus of BPAA-AFF with 10 nm nanofibers self-assembling was around 13.8 kPa,and the morphology was similar to natural extracellular matrix.These supramolecular nanofiber hydrogels could effectively support chondrocytes spreading and proliferation,and specifically enhance chondrogenic related genes expression and chondrogenic matrix secretion.Such biomimetic supramolecular short peptide biomaterials hold great potential in regenerative medicine as promising innovative matrices because of their simple and regular molecular structure and excellent biological performance.

关 键 词：Supramolecular nanofiber hydrogel Biphenyl-tripeptide self-assemblies Molecular dynamics simulations Hydrogen bond interactions Cartilage tissue engineering 

分 类 号：O64[理学—物理化学]