嗜碱性粒细胞活化试验的临床应用  

Clinical applications of basophil activation test: a review

作　　者：高亚东[1] 张珒珒 刘光辉 Ya-Dong GAO;Jin-Jin ZHANG;Guang-Hui LIU(Department of Allergology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院过敏反应科,武汉430071

出　　处：《医学新知》2022年第2期148-155,共8页New Medicine

摘　　要：嗜碱性粒细胞是参与IgE介导的Ⅰ型超敏反应及2型炎症反应的重要效应细胞之一。嗜碱性粒细胞活化试验模拟体内Ⅰ型超敏反应的过程,利用流式细胞术检测过敏原刺激后嗜碱性粒细胞表面特异性标志物的表达水平,以反映嗜碱性粒细胞的活化程度及状态。嗜碱性粒细胞活化试验作为一种过敏原体外试验,具有安全性好、特异性高等优点,在临床上可用于食物或药物过敏、气道过敏性疾病、膜翅目昆虫毒液过敏和严重过敏反应等的辅助诊断,以及不同过敏原特异性免疫治疗的疗效监测。嗜碱性粒细胞活化试验相关过敏原试剂、操作流程及解读方法尚需进一步标准化,以促进该方法的推广应用。Basophils are one of the most important effector cells involved in IgEmediated type I hypersensitivity and type 2 inflammation. Basophil activation test(BAT)simulates in vivo type I hypersensitivity. Flow cytometry is used in BAT to detect the expression level of specific surface markers on basophils after allergen stimulation, hence reflecting the degree of activation and status of basophils. As an allergen test in vitro, BAT shows good safety and high specificity. In clinical practice, it can be used for the auxiliary diagnosis of diseases such as food or drug allergy, airway allergic diseases, hymenoptera venom allergy and anaphylaxis. In addition, it can help to predict and monitor the efficacy of specific immunotherapy for different allergens. Allergen reagents, operational procedures and interpretation methods need to be further standardized to facilitate the popularization and application of BAT.

关 键 词：嗜碱性粒细胞 嗜碱性粒细胞活化试验 流式细胞术 过敏原 过敏性疾病 

分 类 号：R593.1[医药卫生—内科学] R446.6[医药卫生—临床医学]